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Uroplakin 1B antibody

CAT: 0013-GTR18398424-01Size: 0.1 mLDry Ice: NoHazardous: No
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CAT#:0013-GTR18398424-01Size:0.1 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Uroplakin 1B antibody validated for immunohistochemistry on 76 different Normal Tissues
Product Name Alternative
Tetraspanin-20; Tspan-20; TSPAN20; UP1b; UPIb; UPK1B; Uroplakin-1b
UniProt
O75841
Reactivity
Human
Immunogen
Recombinant fragment (around aa 109-229) of human Uroplakin 1B (UPK1B) protein (exact sequence is proprietary)
Clonality
Monoclonal
Clone
MSVA-734M
Conjugation
Unconjugated
Dilution
1:100-1:200
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: Urinary bladder: A strong membranous and cytoplasmic UPK1b immunostaining should be seen in the urothelium (the staining can be limited to the top cell layers or equally involve all cell layers) . Negative Control: Colon: UPK1b immunostaining should be absent in all cells of the colon mucosa. Cellular Localization: Cell Surface and cytoplasm Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
Tested Applications
IHC
Host or Source
Mouse
Isotype
IgG2b, kappa

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