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BRG1 Antibody / SMARCA4

CAT: 0013-GTR18317214Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18317214Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The SWI-SNF complex is involved in the activation of transcription via the remodeling of nucleosome structure in an ATP-dependent manner. Brm (also designated SNF2A) and Brg-1 (also designated SNF2B) are the ATPase subunits of the mammalian SWI/SNF complex. Brm, Brg-1, Ini1 (integrase interactor 1, also designated SNF5), BAF155 (also designated SRG3) and BAF170 are thought to comprise the functional core of the SWI/SNF complex. Addition of Ini1, BAF155 and BAF170 to Brg-1 appears to increase remodeling activity. Other complex subunits are thought to play regulatory roles. hSNF2L and hSNF2H both appear to be homologs of Drosophila ISWI, a Brm related ATPase that is present in chromatin remodeling complexes other than SWI/ SNF, including the NURF (nucleosome remodeling factor) .
UniProt
P51532
Reactivity
Human
Immunogen
A recombinant partial protein sequence (within amino acids 200-400) from the human protein was used as the immunogen for the BRG1 antibody.
Clonality
Recombinant
Clone
BRG1/7633R
Conjugation
Unconjugated
Field of Research
Epigenetics & Chromatin
Purification
Protein A/G affinity
Dilution
ELISA (Order BSA-free format for coating), Western blot: 1-2ug/ml, Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the BRG1 antibody should be determined by the researcher.
Tested Applications
ELISA, IHC-P, WB
Host or Source
Rabbit
Preservative
1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Isotype
Rabbit IgG, kappa

UniProtKB · P51532

SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4

SMCA4_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P51532
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
SMARCA4
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
SMARCA4
EC number
3.6.4.-
Processing
—
Secondary accessions
B1A8Z4, B1A8Z5, B1A8Z6, B1A8Z7, E9PBR8, O95052, Q9HBD3
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

PTM

AcetylationIsopeptide bondPhosphoproteinUbl conjugation

Molecular function

ActivatorChromatin regulatorHydrolaseRepressorRNA-binding

Coding sequence diversity

Alternative splicing

Ligand

ATP-bindingNucleotide-binding

Domain

Bromodomain

Disease

DeafnessDisease variantIntellectual disability

Biological process

NeurogenesisTranscriptionTranscription regulation

Cellular component

Nucleus

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