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TLE1 antibody

CAT: 0013-GTR18398373-01Size: 0.1 mLDry Ice: NoHazardous: No
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CAT#:0013-GTR18398373-01Size:0.1 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
TLE1 antibody validated for immunohistochemistry on 76 different Normal Tissues
Product Name Alternative
Enhancer of split groucho 1 (ESG1) ; Transducin like enhancer of split 1; Transducin-like enhancer protein 1; Enhancer of split groucho-like protein 1; GRG1; TLE1
UniProt
Q04724
Reactivity
Human
Immunogen
Recombinant human TLE1fragment
Clonality
Monoclonal
Clone
MSVA-856R
Conjugation
Unconjugated
Dilution
1:100-1:200
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: Tonsil: A weak to moderate TLE1 staining should be seen in suprabasal cells of squamous epithelium and in a small subset of lymphocytes in germinal centres. Negative Control: Tonsil: The majority of lymphocytes in germinal centres should be TLE1 negative. Cellular Localization: Nucleus Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
Tested Applications
IHC
Host or Source
Rabbit
Isotype
IgG

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