Biotinylated Human BLAME / SLAMF8 Protein, His, Avitag™

• Background:

  BLAME (B-lymphocyte activator macrophage expressed) is also known as SLAM family member 8 (SLAMF8), CD353. BLAME is a cell surface receptor that is expressed upon activation of macrophages (MΦs) by IFN-γ or bacteria, is a negative regulator of ROS in response to Gram+ and Gram- bacteria. May play a role in B-lineage commitment and/or modulation of signaling through the B-cell receptor. SLAMF8 is a costimulatory molecule that affects the activation of macrophages in inflammation and in immunosuppression and inflammation response to glioma cells could aid immunotherapy for glioma.

• Description:

  Biotinylated Human BLAME, His, Avitag (BLE-H82E4) is expressed from human 293 cells (HEK293) . It contains AA Ala 23 - Asp 233 (Accession # Q9P0V8-1) .

• Specifications:

  This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 27.1 kDa. The protein migrates as 30-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

• Accession Number:

  Q9P0V8-1

• Expression Region:

  Ala 23 - Asp 233

• Host:

  HEK293

• Target:

  BLAME

• Conjugation:

  Biotin-labeled

• Tag:

  C-10xHis & C-Avi

• Source:

  Human

• Stability:

  ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

• Endotoxin:

  1.0 EU per μg

• Purity:

  85%

• Bioactivity:

  Immobilized Human BLAME, His Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Human BLAME, His,Avitag (Cat. No. BLE-H82E4) with a linear range of 1-31 ng/mL (QC tested).

• Format:

  Powder

• Buffer:

  50 mM Sodium Citrate, 150 mM NaCl, pH5.5

• Additives:

  Trehalose

• Molecular Weight:

  27.1 kDa

• Shipping Conditions:

  RT

• Storage Conditions:

  -20°C

• Package Size:

  200ug*1

• Species:

  Human