Biotinylated Human BLAME / SLAMF8 Protein, His,Avitag™

Biotinylated Human BLAME / SLAMF8 Protein, His,Avitag™

• Background: BLAME (B-lymphocyte activator macrophage expressed) is also known as SLAM family member 8(SLAMF8), CD353. BLAME is a cell surface receptor that is expressed upon activation of macrophages (MΦs) by IFN-γ or bacteria, is a negative regulator of ROS in response to Gram+ and Gram- bacteria. May play a role in B-lineage commitment and/or modulation of signaling through the B-cell receptor. SLAMF8 is a costimulatory molecule that affects the activation of macrophages in inflammation and in immunosuppression and inflammation response to glioma cells could aid immunotherapy for glioma.
• Description: Biotinylated Human BLAME, His,Avitag (BLE-H82E4) is expressed from human 293 cells (HEK293). It contains AA Ala 23 - Asp 233 (Accession # Q9P0V8-1).
• Specifications: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 27.1 kDa. The protein migrates as 30-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression Region: Ala 23 - Asp 233
• Host: HEK 293
• Target: BLAME
• Conjugation: Biotin-labeled
• Tag: C-10×His & C-Avi
• Source: Human
• Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Endotoxin: 1.0 EU per μg
• Purity: 85%
• Bioactivity: Immobilized Human BLAME, His Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Human BLAME, His,Avitag (Cat. No. BLE-H82E4) with a linear range of 1-31 ng/mL (QC tested).
• Format: Powder
• Buffer: 50 mM Sodium Citrate, 150 mM NaCl, pH5.5
• Additives: Trehalose
• Molecular Weight: 27.1 kDa
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C