Phospho-MST1/MST2 (T180 + T183) Recombinant Rabbit Monoclonal Antibody [JE46-64]

Phospho-MST1/MST2 (T180 + T183) Recombinant Rabbit Monoclonal Antibody [JE46-64]

• Description: The human serine/threonine kinase, mammalian STE20 like kinase (MST), is considerably homologous to the budding yeast kinases, SPS1 and STE20, throughout their kinase domains. When stably expressed in HeLa cells, MST highly sensitizes the cells to death receptor mediated apoptosis by accelerating caspase 3 activation. These findings suggest that MST1 and MST2 play a role in apoptosis both upstream and downstream of caspase activation. MST1 is cleaved and activated by caspases during apoptosis and is capable of inducing apoptotic morphological changes such as chromatin condensation upon overexpression. Mammalian Sterile 20 Like 2 (MST2) is most similar to the previously identified MST1 protein kinase (78% identity, 88% similarity). Northern analysis indicates that MST2 mRNA is expressed at high levels in adult kidney, skeletal and placental tissues and at very low levels in adult heart, lung, liver and brain tissues. An in vitro kinase assay indicates that MST2 can phosphorylate an exogenous substrate, as well as itself, and phospho amino acid analysis indicates that it is a serine/threonine protein kinase.
• Product Name Alternative: DKFZp686A2068 antibody FLJ90748 antibody KRS1 antibody KRS2 antibody Mammalian STE20 like protein kinase 1 antibody Mammalian STE20 like protein kinase 2 antibody MST 1 antibody MST 2 antibody MST1 antibody MST2 antibody Serine/threonine kinase 3 (STE20 homolog yeast) antibody Serine/threonine kinase 3 (Ste20 yeast homolog) antibody Serine/threonine kinase 3 antibody Serine/threonine kinase 4 antibody Serine/threonine protein kinase 3 antibody Serine/threonine protein kinase 4 antibody Serine/threonine protein kinase Krs 1 antibody Serine/threonine protein kinase Krs 2 antibody STE20 like kinase MST1 antibody STE20 like kinase MST2 antibody STK 3 antibody STK 4 antibody STK3 antibody STK4 antibody YSK3 antibody
• Abbreviation: STK4, KRS2, MST1, STK3, KRS1, MST2
• CAS Number: 9000-83-3
• Swiss Prot: Q13043 Human, Q13188 Human, Q9JI11 Mouse, Q9JI10 Mouse, O54748 Rat,
• Cellular Locus: Cytoplasm, Nucleus.
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Thr183 of human MST1 protein.
• Isotype: IgG
• Clone: JE46-64
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB
• Positive Control: Ramos treated with 1μM Staurosporine for 1 hour cell lysate, NIH/3T3 treated with 1μM Staurosporine for 3 hour cell lysate, C6 treated with 1μM Staurosporine for 3 hour cell lysate.
• Concentration: 1 mg/mL
• Dilution: WB: 1:1,000
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 56 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
• Recombinant Antibody: Yes