Anti-Perforin PE-Cy1537

Anti-Perforin PE-Cy1537

• Background: Perforin is a 70 kDa cytolytic protein with structural and functional similarities to complement component 9 (C9). It is stored in cytoplasmic granules of cytotoxic T cells and NK cells and after its release it forms transmembrane pores in the target cells to kill it. As perforin is a key effector molecule for cell-mediated cytolysis, defects of its gene can cause severe disorders.
• Specifications: The mouse monoclonal antibody dG9 (also known as deltaG9) recognizes perforin, a 70 kDa protein expressed in cytoplasmic granules of cytotoxic T cells and NK cells.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Human, Cow
• Negative Species: Mouse
• Immunogen: purified granules from human YT lymphoma cell line
• Target Antigen: Perforin
• Isotype: IgG2b κ
• Clone: dG9
• Applications: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 mL) is sufficient for 100 tests.
• Validated Applications: FC
• Format: PE-Cy1537
• Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Vargas-Inchaustegui DA, Demberg T, Robert-Guroff M: A CD8945(-) subpopulation of macaque circulatory natural killer cells can mediate both antibody-dependent and antibody-independent cytotoxic activities. Immunology. 2011 Nov134(3):326-40., URL: http://www.ncbi.nlm.nih.gov/pubmed/21978002,*Takeuchi M, Inoue T, Otani T, Yamasaki F, Nakamura S, Kibata M: Cell-in-cell structures formed between human cancer cell lines and the cytotoxic regulatory T-cell line HOZOT. J Mol Cell Biol. 2010 Jun2(3):139-51., URL: http://www.ncbi.nlm.nih.gov/pubmed/20348186 ,*Qiu Y, Chen J, Liao H, Zhang Y, Wang H, Li S, Luo Y, Fang D, Li G, Zhou B, Shen L, Chen CY, Huang D, Cai J, Cao K, Jiang L, Zeng G, Chen ZW: Tim-3-expressing CD4+ and CD8+ T cells in human tuberculosis (TB) exhibit polarized effector memory phenotypes and stronger anti-TB effector functions. PLoS Pathog. 20128(11):e1002984., URL: http://www.ncbi.nlm.nih.gov/pubmed/23144609 ,*Hersperger AR, Makedonas G, Betts MR: Flow cytometric detection of perforin upregulation in human CD8 T cells. Cytometry A. 2008 Nov73(11):1050-7., URL: http://www.ncbi.nlm.nih.gov/pubmed/18615597 ,*Hameed A, Olsen KJ, Cheng L, Fox WM, Hruban RH, Podack ER: Immunohistochemical identification of cytotoxic lymphocytes using human perforin monoclonal antibody. Am J Pathol. 1992 May140(5):1025-30., URL: https://www.ncbi.nlm.nih.gov/pubmed/1374586 ,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.