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Mouse RANKL Protein

CAT: 0749-PRP1114-01Size: 5 µgDry Ice: NoHazardous: No
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CAT#:0749-PRP1114-01Size:5 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
RANKL protein is tumor necrosis factor ligand superfamily member 11, also known as Receptor activator of nuclear factor kappa-B ligand, Osteoprotegerin ligand, TNFSF11, RANKL, TRANCE, OPGL and CD254, is expressed in a variety of cells, including osteoblasts, fibroblasts, activated T-cells and bone marrow stromal cells, is also capable of interacting with a decoy receptor called OPG.Tnfsf11 is involved in a number of fundamental biological processes such as acting as regulator of interactions between T-cells and dendritic cells, the regulation of the T-cell-dependent immune response and enhancing bone-resorption in humoral hypercalcemia of malignancy. It augments the ability of dendritic cells to stimulate naive T-cell proliferation
Description
Mouse RANKL protein, expressed in E. coli
Product Name Alternative
Tumor necrosis factor ligand superfamily member 11; RANKL; CD254; ODF; OPGL; TNFSF11; TRANCE
Gene ID
21943
Accession Number
O35235
Tag
RANKL
Type
Cytokines and proteins
Applications
No tag
Purity
≥ 98 % as determined by SDS-PAGE
Activity
Immobilized Mouses RANKL at 1μg/mL (100μl/well) can bind Human OPG (C-Fc), The EC50 of Human OPG is 55 ng/mL.
Molecular Weight
19 kDa
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits
Endotoxin: < 1 EU per μg of the protein as determined by the LAL method
Shipping Conditions
Gel pack with blue ice.
Storage Conditions
Lyophilized Mouse RANKL proteinshould be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) . Please prevent freeze-thaw cycles.
Protein Length
The recombinant Mouse sRANKL consists of 174 amino acids (N-Met) and has a predicted molecular mass of 19 kDa. The apparent molecular mass of the Mouse sRANKL protein is approximately 19 kDa in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile 20 mM Tris, 150 mM NaCl pH 8.0.
Applications Notes
Mouse
Other Product Names
Tumor necrosis factor ligand superfamily member 11; RANKL; CD254; ODF; OPGL; TNFSF11; TRANCE
Recommended Usage
Always centrifuge tubes before opening . It is recommended to centrifuge at 12,000 rpm for 1 minute. Then reconstitute the lyophilized Mouse sRANKL protein with the buffer we provided not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Preparation
E. coli
Accession Number URL
https://www.uniprot.org/uniprot/O35235
AA Sequence
Amino acid sequence derived from Mouse RANKL soluble form (O35235) (Pro143-Asp316) was expressed

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