Mouse anti FITC (Fluorescein isothiocyanate)

• Background:

  The F4/1 antibody recognizes both the free and protein-conjugated (either soluble or cell bound) form of fluorescein or carboxyfluorescein. Sensitivity of the FITC-anti- FITC system is reportedly similar to that of the biotin-avidin system. When conjugated to biotin or enzyme-labeled, this antibody is also suitable for the conversion of fluorescein-labeled detection into immunoenzymatic assays.

• Host:

  Mouse

• Isotype:

  IgG1

• Clone:

  F4/1

• Conjugation:

  FITC

• Type:

  Monoclonal Antibody

• Source:

  F4/1 is a hybridoma formed by fusing SP-2/0 Agl4 cells with spleen cells of a BALB/c mouse that had been immunised with FITC-KLH.

• Applications:

  WB, ELISA, Flow Cytometry

• Field of Research:

  Ancillary Reagents

• Purification Method:

  Protein A/G Chromatography

• Assay Principle:

  This antibody can be used for Western blotting (1-2 µg/mL), immunohistochemistry (frozen and formalin/paraffin tissues, 2-4 µg/mL) and flow cytometry. Optimal concentration should be evaluated by serial dilutions.

• Stability:

  See expiration date on vial

• Concentration:

  See vial for Concentration

• Form:

  Provided as solution in phosphate buffered saline with 0,08% sodium azide

• Precautions:

  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.

• References & Citations:

  1. Balogh P; et al. 'Hapten mediated identification of cell membrane antigens using an anti-FITC monoclonal antibody.' J. Immunol. Methods 1994, 169, 35-40.

• Shipping Conditions:

  Ambient Temperature, freeze upon arrival

• Storage Conditions:

  Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles

• Functional Analysis:

  WB

• CAS Number:

  9007-83-4