EEA1 Mouse Monoclonal Antibody [B0-A8]

EEA1 Mouse Monoclonal Antibody [B0-A8]

• Description: EEA1 localizes exclusively to early endosomes?and has an important role in endosomal trafficking. EEA1 binds directly to the phospholipid phosphatidylinositol 3-phosphate?through its C-terminalFYVE domain?and forms a homodimer?through a?coiled coil. EEA1 acts as a tethering molecule that couples vesicle docking with SNAREs such as?N-ethylmaleimide sensitive fusion protein, bringing the endosomes physically closer and ultimately resulting in the fusion and delivery of endosomal cargo. Due to the proteins importance in vesicular trafficking, a number of intracellular bacteria prevent EEA1 recruitment to the vacuole.Mycobacterium tuberculosis?is known to inhibit the recruitment of EEA1 to the phagosomal membrane through CamKII. Legionella pneumophila?also prevents EEA1 recruitment through a currently unknown mechanism.[5] Interestingly, the related pathogen Legionella longbeachae?recruits EEA1 and appears to replicate within a modified early endosome.
• Product Name Alternative: Early endosome antigen 1 antibody Early endosome antigen 1, 162kD antibody Early endosome associated protein antibody EEA 1 antibody EEA1 antibody EEA1_HUMAN antibody Endosome associated protein p162 antibody Endosome-associated protein p162 antibody MST105 antibody MSTP105 antibody ZFYVE2 antibody Zinc finger FYVE domain containing protein 2 antibody Zinc finger FYVE domain-containing protein 2 antibody
• Abbreviation: EEA1
• CAS Number: 9007-83-4
• Swiss Prot: Q15075 Human, Q8BL66 Mouse, A0A0G2K051 Rat
• Cellular Locus: Cytoplasm, early endosome membrane.
• Host: Mouse
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic peptide of the N terminal residues of Human EEA1.
• Isotype: IgG1
• Conjugation: Non-conjugated
• Type: Mouse monoclonal Antibody
• Applications: WB, IHC-P
• Positive Control: 293, human colon cancer tissue, mouse prostate tissue.
• Concentration: 2 mg/mL
• Dilution: WB: 1:2,000-1:10,000 ;IHC-P: 1:50-1:200
• Purity: Immunogen affinity purified.
• Form: Liquid
• Buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 162 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
• Recombinant Antibody: No