Pyruvate Dehydrogenase E2 Recombinant Rabbit Monoclonal Antibody [JE34-38]

Pyruvate Dehydrogenase E2 Recombinant Rabbit Monoclonal Antibody [JE34-38]

• Description: Dihydrolipoyl transacetylase (or dihydrolipoamide acetyltransferase) is an enzyme component of the multienzyme pyruvate dehydrogenase complex. The pyruvate dehydrogenase complex is responsible for the pyruvate decarboxylation step that links glycolysis to the citric acid cycle. This involves the transformation of pyruvate from glycolysis into acetyl-CoA which is then used in the citric acid cycle to carry out cellular respiration. There are three different enzyme components in the pyruvate dehydrogenase complex. Pyruvate dehydrogenase (EC 1.2.4.1) is responsible for the oxidation of pyruvate, dihydrolipoyl transacetylase (this enzyme; EC 2.3.1.12) transfers the acetyl group to coenzyme A (CoA), and dihydrolipoyl dehydrogenase (EC 1.8.1.4) regenerates the lipoamide. Because dihydrolipoyl transacetylase is the second of the three enzyme components participating in the reaction mechanism for conversion of pyruvate into acetyl CoA, it is sometimes referred to as E2. In humans, dihydrolipoyl transacetylase enzymatic activity resides in the pyruvate dehydrogenase complex component E2 (PDCE2) that is encoded by the DLAT (dihydrolipoamide S-acetyltransferase) gene.
• Product Name Alternative: 70 kDa mitochondrial autoantigen of primary biliary cirrhosis antibody Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex antibody Dihydrolipoamide S Acetyltransferase antibody Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex antibody dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex mitochondrial antibody DLAT antibody DLTA antibody E2 antibody E2 component of pyruvate dehydrogenase complex antibody M2 antigen complex 70 kDa subunit antibody mitochondrial antibody ODP2_HUMAN antibody PBC antibody PDC E2 antibody PDC-E2 antibody PDCE2 antibody Pyruvate dehydrogenase complex component E2 antibody Pyruvate dehydrogenase complex E2 subunit antibody
• Abbreviation: DLAT, DLTA
• CAS Number: 9000-83-3
• Swiss Prot: P10515 Human, Q8BMF4 Mouse, P08461 Rat
• Cellular Locus: Mitochondrion matrix.
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic peptide within human Pyruvate Dehydrogenase E2 aa 574-624/647.
• Isotype: IgG
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IHC-P
• Positive Control: HL-60 cell lysate, Mouse heart tissue lysate, SK-Br-3 cell lysate, K562 cell lysate, Daudi cell lysate, human testis tissue, mouse cerebellum tissue, rat brain tissue.
• Concentration: 1 mg/mL
• Dilution: WB: 1:1,000-1:2,000 ;IHC-P: 1:200
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 69 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
• Recombinant Antibody: Yes