Ki67 Recombinant Rabbit Monoclonal Antibody [PSH0-02]

Ki67 Recombinant Rabbit Monoclonal Antibody [PSH0-02]

• Description: Antigen KI-67, also known as Ki-67, Ki-67 or MKI67 (Marker Of Proliferation Ki-67), is a protein that in humans is encoded by the MKI67 gene (antigen identified by monoclonal antibody Ki-67). Antigen KI-67 is a nuclear protein that is associated with cellular proliferation. Altering Ki-67 expression levels did not significantly affect cell proliferation in vivo. Ki-67 mutant mice developed normally and cells lacking Ki-67 proliferated efficiently. Furthermore, it is associated with ribosomal RNA transcription. Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis. The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation, and can be used in immunohistochemistry. It is strictly associated with cell proliferation. During interphase, the Ki-67 antigen can be exclusively detected within the cell nucleus, whereas in mitosis most of the protein is relocated to the surface of the chromosomes. Ki-67 protein is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent in resting (quiescent) cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. In breast cancer Ki67 identifies a high proliferative subset of patients with ER-positive breast cancer who derive greater benefit from adjuvant chemotherapy. Ki-67 is an excellent marker to determine the growth fraction of a given cell population. The fraction of Ki-67-positive tumor cells (the Ki-67 labeling index) is often correlated with the clinical course of cancer. The best-studied examples in this context are prostate, brain and breast carcinomas, as well as nephroblastoma and neuroendocrine tumors. For these types of tumors, the prognostic value for survival and tumor recurrence have repeatedly been proven in uni- and multivariate analysis.
• Product Name Alternative: Antigen identified by monoclonal antibody Ki 67 antibody Antigen identified by monoclonal antibody Ki-67 antibody Antigen KI-67 antibody Antigen KI67 antibody Antigen Ki67 antibody KI67_HUMAN antibody KIA antibody Marker of proliferation Ki-67 antibody MIB 1 antibody MIB antibody MKI67 antibody PPP1R105 antibody Proliferation marker protein Ki-67 antibody Proliferation related Ki 67 antigen antibody Protein phosphatase 1 regulatory subunit 105 antibody RP11-380J17.2 antibody
• Abbreviation: MKI67
• CAS Number: 9000-83-3
• Swiss Prot: P46013 Human
• Cellular Locus: Chromosome, Nucleus, nucleolus.
• Host: Rabbit
• Species Reactivity: Human
• Immunogen: Synthetic peptide within human Ki67 aa 1000-1200.
• Isotype: IgG
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: IHC-P, FC
• Positive Control: Human cervix carcinoma tissue, human colon carcinoma tissue, human lymph nodes tissue, A431, Hela.
• Concentration: 1 mg/mL
• Dilution: IHC-P: 1:2,000 ;FC: 1:500-1:1,000
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 359 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
• Recombinant Antibody: Yes