HRP Conjugated beta Actin Recombinant Mouse Monoclonal Antibody [A2-F6-R]

HRP Conjugated beta Actin Recombinant Mouse Monoclonal Antibody [A2-F6-R]

• Description: Beta-actin (human gene and protein abbreviation ACTB/ACTB) is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus. Beta-actin has been shown to interact with SPTBN2. In addition, RNA-binding protein Sam68 was found to interact with the mRNA encoding β-actin, which regulates the synaptic formation of the dendritic spines with its cytoskeletal components. Beta-actin has been shown to activate eNOS, thereby increasing NO production. An eight-amino acid residue (326-333) in actin has been shown to mediate the interaction between actin and eNOS. Recurrent mutations in this gene have been associated to cases of diffuse large B-cell lymphoma. Beta actin is often used in Western blotting as a loading control, to normalize total protein amounts and check for eventual protein degradation in the samples. Its transcript is also commonly used as a housekeeping gene standard in qPCR. Its molecular weight is approximately 42 kDa.
• Product Name Alternative: A26C1A antibody A26C1B antibody ACTB antibody ACTB_HUMAN antibody Actin beta antibody Actin cytoplasmic 1 antibody Actin, cytoplasmic 1, N-terminally processed antibody Actx antibody b actin antibody Beta cytoskeletal actin antibody Beta-actin antibody BRWS1 antibody E430023M04Rik antibody MGC128179 antibody PS1TP5 binding protein 1 antibody PS1TP5BP1 antibody β-actin antibody β actin antibody
• Abbreviation: ACTB
• CAS Number: 9000-83-3
• Swiss Prot: P60709 Human, P60710 Mouse, P60711 Rat
• Cellular Locus: Cytoplasm.
• Host: Mouse
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic peptide within N-terminal human Beta-actin .
• Isotype: IgG1
• Conjugation: HRP-conjugated
• Type: Recombinant Mouse monoclonal Antibody
• Applications: WB
• Positive Control: HepG2 cell lysates, NIH/3T3 cell lysates, PC-12 cell lysates, THP-1 cell lysate, MCF-7 cell lysate, RH-35 cell lysate, L6 cell lysate, MEF cell lysate, HepG2 cell lysate, PC-12 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, A549 cell lysate, PMVEC cell lysate.
• Concentration: 0.5 mg/mL
• Dilution: WB: 1:2,500-1:20,000
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol.
• Molecular Weight: Predicted band size: 42 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
• Recombinant Antibody: Yes