beta Actin Mouse Monoclonal Antibody [A2-F6]

beta Actin Mouse Monoclonal Antibody [A2-F6]

• Description: Beta-actin (human gene and protein abbreviation ACTB/ACTB) is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins[5][6] that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus. Beta-actin has been shown to interact with SPTBN2. In addition, RNA-binding protein Sam68 was found to interact with the mRNA encoding β-actin, which regulates the synaptic formation of the dendritic spines with its cytoskeletal components. Beta-actin has been shown to activate eNOS, thereby increasing NO production. An eight-amino acid residue (326-333) in actin has been shown to mediate the interaction between actin and eNOS. Recurrent mutations in this gene have been associated to cases of diffuse large B-cell lymphoma. Beta actin is often used in Western blotting as a loading control, to normalize total protein amounts and check for eventual protein degradation in the samples. Its transcript is also commonly used as a housekeeping gene standard in qPCR. Its molecular weight is approximately 42 kDa.
• Product Name Alternative: A26C1A antibody A26C1B antibody ACTB antibody ACTB_HUMAN antibody Actin beta antibody Actin cytoplasmic 1 antibody Actin, cytoplasmic 1, N-terminally processed antibody Actx antibody b actin antibody Beta cytoskeletal actin antibody Beta-actin antibody BRWS1 antibody E430023M04Rik antibody MGC128179 antibody PS1TP5 binding protein 1 antibody PS1TP5BP1 antibody β-actin antibody β actin antibody
• Abbreviation: ACTB
• CAS Number: 9007-83-4
• Swiss Prot: P60709 Human, P60710 Mouse, P60711 Rat
• Cellular Locus: Cytoplasm.
• Host: Mouse
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic peptide (KLH-coupled) within human Beta-actin aa 1-50.
• Isotype: IgG1
• Conjugation: Non-conjugated
• Type: Mouse monoclonal Antibody
• Applications: WB, IF-Cell, IHC-P, FC
• Positive Control: Hela cell lysate, THP-1 cell lysate, HepG2 cell lysate, MCF-7 cell lysate, PC-12 cell lysate, PMVEC cell lysate, RH-35 cell lysate, L6 cell lysate, NIH/3T3 cell lysate, L929 cell lysate, RAW264.7 cell lysate, B16F1 cell lysate, MEF cell lysate, Hela, A549, NIH/3T3, human colon carcinoma tissue, mouse prostate tissue, mouse kidney tissue.
• Concentration: 2 mg/mL
• Dilution: WB: 1:10,000-160,000 ;IF-Cell: 1:100-1:200 ;IHC-P: 1:1,000 ;FC: 1:1,000
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 42 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Recombinant Antibody: No