Ferritin Light Chain Recombinant Rabbit Monoclonal Antibody [JM10-37]

Ferritin Light Chain Recombinant Rabbit Monoclonal Antibody [JM10-37]

• Description: Mammalian ferritins consist of 24 subunits made up of two types of polypeptide chains, ferritin heavy chain and ferritin light chain, which each have unique functions. Ferritin heavy chains catalyze the first step in iron storage, the oxidation of Fe (II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe (III). The most prominent role of mammalian ferritins is to provide iron-buffering capacity to cells. In addition to iron buffering, heavy chain ferritin is also involved in the regulation of thymidine biosynthesis via increased expression of cytoplasmic serine hydroxymethyltransferase, which is a limiting factor in thymidylate synthesis in MCF-7 cells. Light chain ferritin is involved in cataracts by at least two mechanisms, hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed, and oxidative stress, an important factor in the development of ageing-related cataracts. The gene encoding human ferritin heavy chain maps to chromosome 11q13 and the human ferritin light chain gene maps to chromosome 19q13.3-q13.4.
• Product Name Alternative: Ferritin L chain antibody Ferritin L subunit antibody Ferritin light chain antibody Ferritin light polypeptide antibody ferritin light polypeptide like 3 antibody FRIL_HUMAN antibody FTL antibody LFTD antibody NBIA 3 antibody NBIA3 antibody
• Abbreviation: Ferritin L subunit, FTL
• CAS Number: 9000-83-3
• Swiss Prot: P02792 Human, P29391 Mouse, P02793 Rat
• Cellular Locus: Cytosol, extracellular exosome, extracellular region, autolysosome, azurophil granule lumen, cytoplasm, intracellular ferritin complex, membrane.
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic peptide within Human Ferritin Light Chain aa 126-175 / 175.
• Isotype: IgG
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IHC-P, IP
• Positive Control: Rat liver tissue lysates, human liver tissue, human brain tissue, human kidney tissue, mouse testis tissue, mouse kidney tissue.
• Concentration: 1 mg/mL
• Dilution: WB: 1:1,000-1:5,000 ;IHC-P: 1:100-1:500 ;IP: Use at an assay dependent concentration.
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 20 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Recombinant Antibody: Yes