Lambda Light Chain Recombinant Rabbit Monoclonal Antibody [PD00-56]

• Description:

  While benign (reactive) B-lymphocytic populations produce Ig-molecules containing an almost equal amount of kappa and lambda light chains, i.e. the number of cells producing kappa is more or less equal to the number producing lambda, neoplastic B-lymphocytic populations has light chain restriction (i.e., are monoclonal) producing either kappa or lambda. Consequently, demonstration of light chains is the most important procedure in the diagnosis of neoplasms of B-lymphocytes (lymphomas and leukemias). The amount of Ig produced in individual types of B-lymphocytic neoplasia vary and is often small, demanding a sensitive technique for detection. About 80% of non-Hodgkin lymphomas are B-cell lymphomas, of which the large majority express surface (s) IgM, although the expression in small cell lymphoma/chronic lymphocytic leukaemia is weak. Plasmacytoma/multiple myeloma do not show sIgM. Lymphoplasmacytic lymphoma most often shows sIgM, while plasmacytoma/multiple myeloma show sIgG in 50% and sIgA in 20%. Light chain restriction is the single most important marker for neoplasms of B-lymphocytic origin. Demonstration of heavy chains can sometime be of aid in the study of malignant lymphomas, as lymphoplasmacytic lymphomas are usually focally IgM positive, while plasmacytoma/multiple myeloma in most cases express strong cytoplasmic IgG or IgA. Precursor B-cell neoplasms are are Ig negative. Normal tonsil is appropriate control tissue: approximately 50% of the mantle zone B-cells should show a distinct membrane staining reaction, while the rest should be unstained.

• Product Name Alternative:

  Constant region of lambda light chains antibody Ig lambda chain C regions antibody IGLC antibody Immunoglobulin lambda constant 1 antibody Mcg marker antibody

• Abbreviation:

  IGLC1, IGLV1

• CAS Number:

  9000-83-3

• Swiss Prot:

  P0CG04 Human, P01701 Human

• Cellular Locus:

  Secreted, Cell membrane.

• Host:

  Rabbit

• Species Reactivity:

  Human

• Immunogen:

  Recombinant protein within full length native protein.

• Isotype:

  IgG

• Clone:

  PD00-56

• Conjugation:

  Non-conjugated

• Type:

  Recombinant Rabbit monoclonal Antibody

• Applications:

  WB, IHC-P

• Positive Control:

  Human small intestine tissue lysates, human tonsil tissue.

• Concentration:

  1 mg/mL

• Dilution:

  WB: 1:1,000 ;IHC-P: 1:3,000

• Purity:

  Protein A affinity purified.

• Form:

  Liquid

• Buffer:

  PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• Molecular Weight:

  Predicted band size: 11 kDa

• Storage Conditions:

  Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

• Recombinant Antibody:

  Yes