Myosin heavy chain Recombinant Rabbit Monoclonal Antibody [JF097-7]

Myosin heavy chain Recombinant Rabbit Monoclonal Antibody [JF097-7]

• Description: Myosin is a highly conserved, ubiquitously expressed protein that interacts with Actin to generate the force for cellular movements. Conventional myosins are hexameric proteins consisting of two heavy chain subunits, a pair of non-phosphorylatable light chain subunits and a pair of phosphorylatable light chain subunits. Three general classes of myosin have been cloned: smooth muscle myosins, striated muscle myosins and non-muscle myosins. Contractile activity in smooth muscle is regulated by the calcium/calmodulin-dependent phosphorylation of myosin light chain by myosin light chain kinase. Myosin heavy chains are encoded by the MYH gene family and have Actin-activated ATPase activity which generates the motor function of myosin. Myosin heavy chains, which were initially isolated from a human fetal skeletal muscle, are the major determinant in the speed of contraction of skeletal muscle. Various isoforms of myosin heavy chain are differentially expressed depending on the functional activity of the muscle.
• Product Name Alternative: cardiac muscle alpha isoform antibody MYH6 antibody MYH6_HUMAN antibody MYH7 antibody MYHC antibody MyHC-alpha antibody MyHC-beta antibody MYHCA antibody MYHCB antibody Myosin heavy chain 6 antibody Myosin heavy chain antibody Myosin heavy chain cardiac muscle alpha isoform antibody Myosin heavy chain cardiac muscle beta isoform antibody Myosin-6 antibody
• Abbreviation: MyHC-alpha, MYH6
• CAS Number: 9000-83-3
• Swiss Prot: P13533 Human, P12883 Human, Q02566 Mouse, P02563 Rat
• Cellular Locus: Cytoplasm, myofibril.
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant protein within Human Myosin heavy chain aa 1220-1420 / 1939.
• Isotype: IgG
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IHC-P, IF-Tissue
• Positive Control: Human skeletal muscle tissue lysates, rat skeletal muscle tissue, rat heart tissue, mouse smooth muscle tissue, mouse skeletal muscle tissue, mouse heart tissue, rat skeletal muscle tissue lysates, human striated muscle tissue, human heart tissue, mouse heart tissue.
• Concentration: 1 mg/mL
• Dilution: WB:1:500-1:2,000 ;IHC-P:1:100-1:500 ;IF-Tissue: 1:100
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 224 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
• Recombinant Antibody: Yes