DKK1 Recombinant Rabbit Monoclonal Antibody [SC06-86]

• Description:

  The Wnt genes are a group of well conserved, cysteine-rich secreted glycoproteins that are required for numerous developmental processes including embryogenesis, asymmetric cell division and central nervous system (CNS) patterning. Wnt association with the seven membrane spanning receptor frizzled activates dishevelled, which downregulates glycogen synthase kinase (GSK) through serine phosphorylation, causing the accumulation of b-catenin and subsequent regulation of developmentally significant Wnt |target genes. The Dickkopf family of secreted inhibitors of Wnt signaling ensures proper morphological development by antagonizing different stages of the Wnt cascade. Dkk-1 (Dickkopf-1) acts upstream of b-catenin and dishevelled to inhibit Wnt signaling. Dkk-1 is a 266-amino acid (human), secreted protein that contains a 31-residue N-terminal signal peptide, 2 cysteine rich domains, and a putative carboxy terminal N-glycosylation site. Human Dkk-1 transcripts are abundantly present in fetal kidney, adult placenta and adult prostate. Putative cis regulatory elements upstream of the Dkk-1 start site include p53, Sp1, MyoD, STAT, Oct-1/2, C/EBP-a, C/EBP-b, GATA-1, GATA-2 and GATA-3.

• Product Name Alternative:

  Dickkopf 1 antibody Dickkopf 1 homolog antibody Dickkopf 1 like antibody Dickkopf homolog 1 antibody Dickkopf like protein 1 antibody Dickkopf related protein 1 antibody Dickkopf WNT signaling pathway inhibitor 1 antibody Dickkopf-1 antibody Dickkopf-related protein 1 antibody DKK 1 antibody Dkk-1 antibody Dkk1 antibody DKK1_HUMAN antibody hDkk 1 antibody hDkk-1 antibody SK antibody

• Abbreviation:

  Dickkopf-1, Dkk-1, hDkk-1, DKK1

• Swiss Prot:

  O94907 Human

• Cellular Locus:

  Secreted.

• Host:

  Rabbit

• Species Reactivity:

  Human, Rat

• Immunogen:

  Synthetic peptide within Human DKK1 aa 225-266 / 266.

• Isotype:

  IgG

• Conjugation:

  Non-conjugated

• Type:

  Recombinant Rabbit monoclonal Antibody

• Applications:

  WB, IF-Cell, IF-Tissue, IHC-P, FC

• Positive Control:

  Caco-2 cell lysates, Hela, HepG2, NCCIT, human uterus tissue, human liver tissue.

• Concentration:

  1 mg/mL

• Dilution:

  WB: 1:2,000 ;IF-Cell: 1:50-1:500 ;IF-Tissue: 1:50-1:500 ;IHC-P: 1:50-1:1,000 ;FC: 1:50-1:100

• Purity:

  Protein A affinity purified.

• Form:

  Liquid

• Buffer:

  1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• Molecular Weight:

  Predicted band size: 29 kDa

• Storage Conditions:

  Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

• Recombinant Antibody:

  Yes

• CAS Number:

  9000-83-3