Cytochrome C Recombinant Rabbit Monoclonal Antibody [SC69-08]

Cytochrome C Recombinant Rabbit Monoclonal Antibody [SC69-08]

• Description: Cytochrome c is a well characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial intermembrane space. More recent studies have identifed cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is translocated from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3.
• Product Name Alternative: CYC antibody CYC_HUMAN antibody CYCS antibody Cytochrome c antibody Cytochrome c somatic antibody HCS antibody THC4 antibody
• Abbreviation: CYCS
• CAS Number: 9000-83-3
• Swiss Prot: P99999 Human, P62897 Mouse, P62898 Rat
• Cellular Locus: Mitochondrion intermembrane space.
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic peptide within human Cytochrome C aa 20-60.
• Isotype: IgG
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IF-Tissue, IHC-P, IP, IF-Cell
• Positive Control: HeLa cell lysate, C6 cell lysate, Mouse brain tissue lysate, Mouse kidney tissue lysate, Rat brain tissue lysate, Rat kidney tissue lysate, Jurkat cell lysate, Human kidney tissue lysate, C6, human kidney tissue, mouse liver tissue, mouse kidney tissue, rat brain tissue.
• Concentration: 1 mg/mL
• Dilution: WB: 1:2,000 ;IF-Tissue: 1:50-1:100 ;IHC-P: 1:1,000-1:5,000 ;IP: Use at an assay dependent concentration. ;IF-Cell: 1:100
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 12 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Recombinant Antibody: Yes