Myosin light chain kinase Recombinant Rabbit Monoclonal Antibody [SU40-06]

Myosin light chain kinase Recombinant Rabbit Monoclonal Antibody [SU40-06]

• Description: MLCK, a member of the Ser/Thr protein kinase family, is a calcium/calmodulin-dependent enzyme responsible for smooth muscle contraction via phosphorylation of a specific serine in the N-terminus of myosin light chains (MLC), an event that facilitates myosin interaction with actin filaments. It is a central determinant in the development of vascular permeability and tissue edema formation. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. MLCK acts as a critical participant in signaling sequences that result in fibroblast apoptosis. Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. The Telokin isoform, which binds calmodulin, has been found in a wide variety of adult and fetal tissues. MLCK is probably down-regulated by phosphorylation. The protein contains 1 fibronectin type III domain and 9 immunoglobulin-like C2-type domains.
• Product Name Alternative: deglutamylated form antibody DKFZp686I10125 antibody EC 2.7.11.18 antibody FLJ12216 antibody Kinase related protein antibody Kinase-related protein antibody KRP antibody MLCK antibody MLCK1 antibody MLCK108 antibody MLCK210 antibody MSTP083 antibody MYLK antibody MYLK_HUMAN antibody MYLK1 antibody Myosin light chain kinase antibody Myosin light polypeptide kinase antibody OTTHUMP00000180642 antibody OTTHUMP00000180643 antibody smMLCK antibody smooth muscle antibody Smooth muscle myosin light chain kinase antibody Telokin antibody
• Abbreviation: MLCK, smMLCK, KRP, MYLK
• CAS Number: 9000-83-3
• Swiss Prot: Q15746 Human, Q6PDN3 Mouse, D3ZFU9 Rat
• Cellular Locus: Cytoplasm, Cell projection, Cleavage furrow, Cytoskeleton.
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic peptide within Human MYLK aa 1,865-1,914 / 1,914.
• Isotype: IgG
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IF-Tissue, IHC-P
• Positive Control: Rat spleen tissue lysates, Human lung tissue lysates, rat smooth muscle tissue, human colon carcinoma tissue, mouse smooth muscle tissue.
• Concentration: 1 mg/mL
• Dilution: WB: 1:1,000-1:10,000 ;IF-Tissue: 1:100-1:500 ;IHC-P: 1:100-1:800
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 211 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Recombinant Antibody: Yes