NADPH oxidase 4/NOX4 Recombinant Rabbit Monoclonal Antibody [SY0214]

NADPH oxidase 4/NOX4 Recombinant Rabbit Monoclonal Antibody [SY0214]

• Description: The superoxide-generating NADPH oxidase includes a membrane-bound flavocytochrome containing two subunits, gp91-phox and p22-phox, and the cytosolic proteins p47-phox and p67-phox. During activation of the NADPH oxidase, p47-phox and p67-phox migrate to the plasma membrane where they associate with the flavocytochrome, cytochrome b558, to form the active enzyme complex. The p22 and gp91-phox subunits also function as surface O2 sensors that initiate cellular signaling in response to hypoxic conditions. Nox4 (also known as Renox) is a renal gp91-phox homolog highly expressed at the site of erythropoietin production in the proximal convoluted tubule epithelial cells of the renal cortex. Nox4 is also expressed in fetal tissues, placenta, glioblastoma and vascular cells. Like gp91-phox, the enzymatic activity of Nox4 produces superoxide anions. In vascular cells, the addition of Angiotensin II increases Nox4 expression, which suggests a role for Nox4 in vascular oxidative stress response. The gene encoding human Nox4 maps to chromosome 11q14.2-q21.
• Product Name Alternative: Kidney oxidase-1 antibody Kidney superoxide-producing NADPH oxidase antibody KOX 1 antibody KOX antibody Kox-1 antibody NADPH antibody NADPH oxidase 4 antibody Nox4 antibody NOX4_HUMAN antibody Renal NAD (P)H-oxidase antibody RENOX antibody
• Abbreviation: KOX-1, NOX4, RENOX
• CAS Number: 9000-83-3
• Swiss Prot: Q9NPH5 Human, Q924V1 Rat
• Cellular Locus: Cell membrane, Nucleus, Endoplasmic reticulum membrane.
• Host: Rabbit
• Species Reactivity: Human, Rat, Mouse
• Immunogen: Recombinant protein within Human NOX4 aa 460-578 / 578.
• Isotype: IgG
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IF-Cell, IHC-P, IP, FC
• Positive Control: SH-SY5Y cell lysate, U-2 OS cell lysate, SH-SY5Y, human lung tissue, human kidney tissue, rat stomach tissue, rat kidney tissue.
• Concentration: 1 mg/mL
• Dilution: WB: 1:2,000 ;IF-Cell: 1:100 ;IHC-P: 1:50-1:200 ;IP: 1-2μg/sample ;FC: 1:1,000
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 67 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Recombinant Antibody: Yes