Phospho-c-Myc (T58 + S62) Recombinant Rabbit Monoclonal Antibody [SZ02-06]

Phospho-c-Myc (T58 + S62) Recombinant Rabbit Monoclonal Antibody [SZ02-06]

• Description: c-Myc-, N-Myc- and L-Myc-encoded proteins function in cell proliferation, differentiation and neoplastic disease. Myc proteins are nuclear proteins with relatively short half lives. Amplification of the c-Myc gene has been found in several types of human tumors including lung, breast and colon carcinomas, while the N-Myc gene has been found amplified in neuroblastomas. The L-Myc gene has been reported to be amplified and expressed at high level in human small cell lung carcinomas. The presence of three sequence motifs in the c-Myc COOH terminus, including the leucine zipper, the helix-loop-helix and a basic region provided initial evidence for a sequence-specific binding function. A basic region helix-loop-helix leucine zipper motif (bHLH-Zip) protein, designated Max, specifically associates with c-Myc, N-Myc and L-Myc proteins. The Myc-Max complex binds to DNA in a sequence-specific manner under conditions where neither Max nor Myc exhibit appreciable binding. Max can also form heterodimers with at least two additional bHLH-Zip proteins, Mad and Mxi1, and Mad-Max dimers have been shown to repress transcription through interaction with mSin3.
• Product Name Alternative: Avian myelocytomatosis viral oncogene homolog antibody bHLHe39 antibody c Myc antibody Class E basic helix-loop-helix protein 39 antibody MRTL antibody Myc antibody Myc protein antibody Myc proto oncogene protein antibody Myc proto-oncogene protein antibody myc related translation/localization regulatory factor antibody MYC_HUMAN antibody Myc2 antibody MYCC antibody Niard antibody Nird antibody Proto-oncogene c-Myc antibody Transcription factor p64 antibody v myc avian myelocytomatosis viral oncogene homolog antibody v myc myelocytomatosis viral oncogene homolog antibody
• Abbreviation: bHLHe39, MYC
• CAS Number: 9000-83-3
• Swiss Prot: P01106 Human, P01108 Mouse
• Cellular Locus: Nucleus.
• Host: Rabbit
• Species Reactivity: Human, Mouse
• Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Thr58 and Ser62 of Human c-Myc.
• Isotype: IgG
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IP, FC
• Positive Control: HCT 116 cell lysate, HCT 116 treated with 25μM MG-132 for 4 hours cell lysate, EL4 cell lysate, EL4 treated with 25μM MG-132 for 4 hours cell lysate, K562.
• Concentration: 1 mg/mL
• Dilution: WB: 1:1,000-1:5,000 ;FC: 1:50-1:100 ;IP: Use at an assay dependent concentration.
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 49 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Recombinant Antibody: Yes