ATP citrate lyase Rabbit Polyclonal Antibody

ATP citrate lyase Rabbit Polyclonal Antibody

• Description: ATP citrate synthase (also ATP citrate lyase (ACLY)) is an enzyme that in animals represents an important step in fatty acid biosynthesis. By converting citrate to acetyl-CoA, the enzyme links carbohydrate metabolism, which yields citrate as an intermediate, with fatty acid biosynthesis, which consumes acetyl-CoA. In plants, ATP citrate lyase generates cytosolic acetyl-CoA precursors of thousands of specialized metabolites, including waxes, sterols, and polyketides. ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer of apparently identical subunits. In animals, the product, acetyl-CoA, is used in several important biosynthetic pathways, including lipogenesis and cholesterogenesis. It is activated by insulin. In plants, ATP citrate lyase generates acetyl-CoA for cytosolically-synthesized metabolites; Acetyl-CoA is not transported across subcellular membranes of plants. Such metabolites include: elongated fatty acids (used in seed oils, membrane phospholipids, the ceramide moieties of sphingolipids, cuticle, cutin, and suberin); flavonoids; malonic acid; acetylated phenolics, alkaloids, isoprenoids, anthocyanins, and sugars; and, mevalonate-derived isoprenoids (e.g., sesquiterpenes, sterols, brassinosteroids); malonyl and acyl-derivatives (d-amino acids, malonylated flavonoids, acylated, prenylated and malonated proteins). De novo fatty acid biosynthesis in plants occurs in plastids; thus, ATP citrate lyase is not relevant to this pathway.
• Product Name Alternative: ACL antibody Acly antibody ACLY_HUMAN antibody ATP citrate (pro-S) lyase antibody ATP citrate lyase antibody ATP citrate synthase antibody ATP-citrate (pro-S-)-lyase antibody ATP-citrate synthase antibody ATPcitrate synthase antibody ATPCL antibody Citrate cleavage enzyme antibody CLATP antibody OTTHUMP00000164773 antibody
• Abbreviation: ACL, ACLY
• CAS Number: 9007-83-4
• Swiss Prot: P53396 Human, Q91V92 Mouse, P16638 Rat
• Cellular Locus: Cytoplasm, cytosol.
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant protein within human ATP citrate lyase aa 813-1066.
• Isotype: IgG
• Conjugation: Non-conjugated
• Type: Rabbit polyclonal Antibody
• Applications: WB, IF-Cell, IHC-P, FC
• Positive Control: NIH-3T3, A549, SH-SY5Y, mouse pancreas tissue lysate, rat brain tissue, human colon cancer tissue, human pancreas tissue, mouse testis tissue.
• Concentration: 1 mg/mL
• Dilution: WB: 1:500;IF-Cell: 1:50-1:200;IHC-P: 1:50-1:200;FC: 1:50-1:100
• Purity: Immunogen affinity purified.
• Form: Liquid
• Buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 121 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Recombinant Antibody: No