alpha Actinin Mouse Monoclonal Antibody [A1G2]

• Description:

  Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene.

• Product Name Alternative:

  actinin 1 smooth muscle antibody Actinin alpha 1 antibody actinin, alpha 1 antibody ACTN 1 antibody Actn1 antibody ACTN1_HUMAN antibody Alpha Actinin 1 antibody Alpha actinin cytoskeletal isoform antibody Alpha-actinin cytoskeletal isoform antibody Alpha-actinin-1 antibody BDPLT15 antibody F actin cross linking protein antibody F-actin cross-linking protein antibody FLJ40884 antibody FLJ54432 antibody Non muscle alpha actinin 1 antibody Non-muscle alpha-actinin-1 antibody

• Abbreviation:

  ACTN1

• Swiss Prot:

  P12814 Human, Q9Z1P2 Rat

• Cellular Locus:

  Cell membrane, cytoskeleton, Z line, cell junction, ruffle.

• Host:

  Mouse

• Species Reactivity:

  Human, Rat

• Immunogen:

  Recombinant protein within Human ACTN1 aa 388-619 / 892.

• Isotype:

  IgG1

• Conjugation:

  Non-conjugated

• Type:

  Mouse monoclonal Antibody

• Applications:

  WB, IF-Cell, IHC-P, FC

• Positive Control:

  HeLa cell lysate, A431 cell lysate, A549 cell lysate, MCF7 cell lysate, PC-12 cell lysate, C6 cell lysate, A431, SiHa, JAR, human lung tissue, human liver carcinoma tissue, human skin tissue, human breast tissue, human breast carcinoma tissue, human kidney tissue.

• Concentration:

  2 mg/mL

• Dilution:

  WB: 1:1,000 ;IF-Cell: 1:50-1:100 ;IHC-P: 1:50-1:500 ;FC: 1:50-1:100

• Purity:

  Protein A affinity purified.

• Form:

  Liquid

• Buffer:

  1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

• Molecular Weight:

  Predicted band size: 103 kDa

• Storage Conditions:

  Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

• Recombinant Antibody:

  No

• CAS Number:

  9007-83-4