Recombinant Human Proliferating Cell Nuclear Antigen

• Description:

  Recombinant Human Proliferating Cell Nuclear Antigen

• Product Name Alternative:

  PCNA

• Gene Name:

  PCNA

• UniProt:

  P12004

• Cellular Locus:

  Nucleus , Note=Colocalizes with CREBBP, EP300 and POLD1 to sites of DNA damage (PubMed:24939902). Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase (PubMed:15543136). Co-localizes with SMARCA5/SNF2H and BAZ1B/WSTF at replication foci during S phase (PubMed:15543136). Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents.

• Reactivity:

  Human

• Target:

  Proliferating Cell Nuclear Antigen

• Type:

  Protein

• Applications:

  WB

• Concentration:

  Lot Specific

• Dilution:

  Dilute in PBS or medium that is identical to that used in the assay system.

• Form:

  Liquid

• Buffer:

  20 mM Tris

• Function:

  Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed:24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs rPubMed:18719106, PubMed:19443450, PubMed:24695737, PubMed:24939902}.

• Storage Conditions:

  Although stable at 4°C for 4 weeks, product is best stored at or below -20°C. Addition of a carrier protein (such as 0.1% HSA or BSA) is recommended for long-term storage. For in vitro investigational use only.

• Specificity:

  Proliferating Cell Nuclear Antigen

• Formulation:

  Sterile-filtered solution in 20mM Tris, pH 7.5, 2mM EDTA, and 20% glycerol.

• Buffer pH:

  pH 7.5

• Target Background:

  PCNA is located in the nucleus and is a cofactor of DNA polymerase delta. PCNA acts as a homotrimer and helps in processing of leading strand synthesis during DNA replication. In reaction to DNA damage, PCNA is ubiquitinated and participates in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA gene. PCNA is expressed during late G1 phase, S phase of mitosis, and persists until the end of the M phase. PCNA can be induced by UV irradiation.

• CAS Number:

  9000-83-3