Anti-Biotin Purified

Anti-Biotin Purified

• Specifications: Mouse monoclonal antibody 1D4-C5 recognizes biotin and can be used as a second-step reagent for detecting biotinylated antigens.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Immunogen: biotinylated limulus hemocyanin
• Target Antigen: Biotin
• Isotype: IgG2a κ
• Clone: 1D4-C5
• Validated Applications: FC, ELISA, IP, MC-CyTOF
• Concentration: 1 mg/mL
• Format: Purified
• Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Green NM, Moody KS, Debatis M, Marshak-Rothstein A: Activation of autoreactive B cells by endogenous TLR7 and TLR3 RNA ligands. J Biol Chem. 2012 Nov 16287(47):39789-99. , URL: https://pubmed.ncbi.nlm.nih.gov/23019335/,*Uccellini MB, Busconi L, Green NM, Busto P, Christensen SR, Shlomchik MJ, Marshak-Rothstein A, Viglianti GA: Autoreactive B cells discriminate CpG-rich and CpG-poor DNA and this response is modulated by IFN-alpha. J Immunol. 2008 Nov 1181(9):5875-84., URL: https://pubmed.ncbi.nlm.nih.gov/18941176/,*Stensland ZC, Smith MJ: Enrichment and detection of antigen-binding B cells for mass cytometry. Magnetochemistry. 2021 Jul7(7):92., URL: https://pubmed.ncbi.nlm.nih.gov/34295938/,
• Storage Conditions: Store at 2-8°C. Do not freeze.