Anti-SARS-CoV-2 Nucleocapsid Antibody, Mouse IgG1 (AS46) (Trehalose free)

• Background:

  Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology; 517: 62-68) . The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.

• Description:

  Anti-SARS-CoV-2 Nucleocapsid Antibody, Mouse IgG1 (AS46) (NUN-S46) was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified recombinant SARS-CoV-2 Nucleocapsid protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. As verified by binding test with N-NTD (Cat.No. NUN-C5143) and N-CTD (Cat.No. NUN-C5145) protein, this antibody can only bind to N-NTD (AA Gly 44 - Glu 174) .

• Host:

  HEK293

• Origin Species:

  Mouse

• Target:

  Nucleocapsid protein

• Conjugation:

  Unconjugated

• Tag:

  Mouse IgG1 | Mouse Kappa

• Stability:

  ● For long term storage, the product is stable for up to 3 years at -70°C from date of receipt; ● For short term storage, the product is stable for up to 12 months at 2-8°C from date of receipt. Please avoid repeated freeze-thaw cycles.

• Purity:

  95%

• Bioactivity:

  Detection SARS-CoV-2 Nucleocapsid Protein by Sandwich ELISA Assay.
  Immobilized Anti-SARS-CoV-2 Nucleocapsid Antibody, Mouse IgG1 (AS46) (Cat. No. NUN-S46) at 4 μg/mL (100 μL/well) can bind SARS-CoV-2 Nucleocapsid Protein (Cat. No. NUN-C5227). And then add Biotinylated Anti-SARS-CoV-2 Nucleocapsid Antibody, Mouse IgG1 (Cat. No. NUN-S47L8) at 0.05 µg/mL. Detection was performed using high sensitivity HRP-conjugated streptavidin with sensitivity of 12.5 pg/mL (Routinely tested).

• Format:

  Liquid

• Buffer:

  PBS, pH7.4

• Shipping Conditions:

  Blue ice

• Storage Conditions:

  2-8°C

• Package Size:

  100ug*1

• Species:

  Mouse

• CAS Number:

  9007-83-4