SARS-CoV-2 Nucleocapsid protein (P80R), His Tag

• Background:

  Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology; 517: 62-68). The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.

• Description:

  SARS-CoV-2 Nucleocapsid protein (P80R), His Tag (NUN-C52Hc) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 419 (Accession # QHO62115.1 (P80R)). The nucleocapsid protein is consisted of the N-terminal RNA-binding domain (NTD) and the C-terminal dimerization domain (CTD), divided by a central serine/arginine-rich (SR)-linker responsible for phosphorylation. The mutation P80R was identified in the SARS-CoV-2 Gamma variant (Pango lineage: P.1; other names: 20J/501Y.V3) (data retrieved from https://pubmed.ncbi.nlm.nih.gov/33095454/).

• Specifications:

  This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 47.4 kDa. The protein migrates as 60-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

• Expression Region:

  Met 1 - Ala 419

• Host:

  HEK 293

• Target:

  Nucleocapsid protein

• Conjugation:

  Unconjugated

• Tag:

  C-6×His

• Source:

  SARS-CoV-2

• Stability:

  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.

• Endotoxin:

  1.0 EU per μg

• Purity:

  95%

• Bioactivity:

  Immobilized SARS-CoV-2 Nucleocapsid protein (P80R), His Tag (Cat. No. NUN-C52Hc) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Nucleocapsid Antibody, Chimeric mAb, Human IgG1 with a linear range of 0.1-2 ng/mL (QC tested).

• Format:

  Powder

• Buffer:

  PBS, 0.5 M Arginine, pH7.4

• Additives:

  Trehalose

• Molecular Weight:

  47.4 kDa

• Shipping Conditions:

  Room Temperature

• Storage Conditions:

  -20°C