Cynomolgus Integrin alpha V beta 1 (ITGAV&ITGB1) Heterodimer Protein, His Tag&Tag Free

• Background:

  Integrin alpha-5/beta-1 is a receptor for ibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-V/beta-1 is also a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Isoform 2 interferes with isoform 1 resulting in a dominant negative effect on cell adhesion and migration (in vitro) . When associated with alpha-7/beta-1 integrin, regulates cell adhesion and laminin matrix deposition.

• Description:

  Cynomolgus Integrin alpha V beta 1 (ITGAV&ITGB1) Heterodimer Protein, His Tag&Tag Free (IT1-C52W4) is expressed from human 293 cells (HEK293) . It contains AA Phe 31 - Pro 993 & Gln 161 - Asp 868 (Accession # A0A2K5WCD3-1 & A0A7N9D0D7) .

• Specifications:

  Cynomolgus Integrin alpha V beta 1 (ITGAV&ITGB1) Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGAV and ITGB1, has a calculated MW of 113 kDa (ITGAV) and 83.6 kDa (ITGB1) . Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The protein migrates as 110-130 kDa (ITGAV) and 80-100 kDa (ITGB1) when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

• Accession Number:

  A0A2K5WCD3-1 & A0A7N9D0D7

• Expression Region:

  Phe 31 - Pro 993 & Gln 161 - Asp 868

• Host:

  HEK293

• Target:

  Integrin alpha V beta 1

• Conjugation:

  Unconjugated

• Tag:

  C-10xHis | Native

• Source:

  Cynomolgus

• Stability:

  ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

• Endotoxin:

  1.0 EU per μg

• Purity:

  90%

• Format:

  Powder

• Buffer:

  50 mM Tris, 150 mM NaCl, pH8.0

• Additives:

  Trehalose

• Molecular Weight:

  113 kDa & 83.6 kDa

• Shipping Conditions:

  RT

• Storage Conditions:

  -20°C

• Package Size:

  500ug*2

• Species:

  Cynomolgus