Human Complement C5a Protein, His Tag

• Background :

  Complement Component C5a (C5a) is also known as C5, and is a protein fragment released from complement component C5., is a potent chemotactic factor for human peripheral blood neutrophils and monocytes, and is believed to play an important role in a number of inflammatory conditions. There are several functions in the below: C5a is an anaphylatoxin, causing the release of histamine from mast cells; C5a is effective leukocyte chemoattractants, causing the accumulation of white blood cells, especially neutrophil granulocytes, at sites of complement activation; C5a activates white blood cells by increasing avidity for white blood cell integrins and upregulating the Lipoxygenase pathway for arachidonic acid metabolism; C5a is a powerful inflammatory mediator, and seems to be a key factor in the development of pathology of many inflammatory diseases involving the complement system; C5a modulates balance between activating versus inhibitory IgG Fc receptors on leukocytes, thereby enhancing autoimmune response.

• Description :

  Complement Component C5a (C5a) is also known as C5, and is a protein fragment released from complement component C5., is a potent chemotactic factor for human peripheral blood neutrophils and monocytes, and is believed to play an important role in a number of inflammatory conditions. There are several functions in the below: C5a is an anaphylatoxin, causing the release of histamine from mast cells; C5a is effective leukocyte chemoattractants, causing the accumulation of white blood cells, especially neutrophil granulocytes, at sites of complement activation; C5a activates white blood cells by increasing avidity for white blood cell integrins and upregulating the Lipoxygenase pathway for arachidonic acid metabolism; C5a is a powerful inflammatory mediator, and seems to be a key factor in the development of pathology of many inflammatory diseases involving the complement system; C5a modulates balance between activating versus inhibitory IgG Fc receptors on leukocytes, thereby enhancing autoimmune response.

• Specifications :

  This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 10.2 kDa. The protein migrates as 14-15 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) .

• Accession Number :

  P01031-1

• Expression Region :

  Leu 679 - Arg 751

• Host :

  E. coli

• Target :

  Complement C5a

• Conjugation :

  Unconjugated

• Tag :

  N-10xHis

• Source :

  Human

• Stability :

  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

• Endotoxin :

  1.0 EU per μg

• Purity :

  95%

• Bioactivity :

  Immobilized Human Complement C5a, His Tag (Cat. No. C5A-H51H9) at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-Human C5a Human Antibody, Human IgG1 with a linear range of 0.1-4 ng/ml (QC tested).

• Format :

  Powder

• Buffer :

  PBS, pH7.4

• Additives :

  Trehalose

• Molecular Weight :

  10.2 kDa

• Additionnal Information :

  Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments

• Shipping Conditions :

  RT

• Storage Conditions :

  -20°C

• Package Size :

  50ug*1

• Host or Source :

  E. coli

• Species :

  Human

• Protein ID :

  P01031-1

• Preservative :

  Trehalose