Cynomolgus Complement C5a Protein, Tag Free

Cynomolgus Complement C5a Protein, Tag Free

• Background: Complement Component C5a (C5a) is also known as C5,and is a protein fragment released from complement component C5.,is a potent chemotactic factor for human peripheral blood neutrophils and monocytes, and is believed to play an important role in a number of inflammatory conditions. There are several functions in the below: C5a is an anaphylatoxin, causing the release of histamine from mast cells; C5a is effective leukocyte chemoattractants, causing the accumulation of white blood cells, especially neutrophil granulocytes, at sites of complement activation; C5a activates white blood cells by increasing avidity for white blood cell integrins and upregulating the Lipoxygenase pathway for arachidonic acid metabolism; C5a is a powerful inflammatory mediator, and seems to be a key factor in the development of pathology of many inflammatory diseases involving the complement system; C5a modulates balance between activating versus inhibitory IgG Fc receptors on leukocytes, thereby enhancing autoimmune response.
• Description: Cynomolgus Complement C5a, Tag Free (C5A-C5118) is expressed from E. coli cells. It contains AA Met 678 - Arg 751 (Accession # XP_015292262.1).
• Specifications: This protein carries no "tag". The protein has a calculated MW of 8.4 kDa. The protein migrates as 11 kDa under reducing (R) condition (SDS-PAGE).
• Expression Region: Met 678 - Arg 751
• Host: E. coli
• Target: Complement C5a
• Conjugation: Unconjugated
• Tag: Native
• Source: Cynomolgus
• Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Endotoxin: 1.0 EU per μg
• Purity: 95%
• Bioactivity: Immobilized Cynomolgus Complement C5a, Tag Free (Cat. No. C5A-C5118) at 2 μg/mL (100 μL/well) can bind Anti-C5a (Human IgG1) with a linear range of 0.8-13 ng/mL (QC tested).
• Format: Powder
• Buffer: PBS, pH7.4
• Additives: Trehalose
• Molecular Weight: 8.4 kDa
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C