HSV-2 (strain 333) Envelope Glycoprotein D (gD), His Tag (MALS verified)

HSV-2 (strain 333) Envelope Glycoprotein D (gD), His Tag (MALS verified)

• Background: Herpesvirus infections are widely spread throughout the world population. Herpes simplex virus (HSV) belongs to the α-herpesvirus subfamily. There are two main types of HSV, HSV-1 and HSV-2, which infect humans. HSV-2 mainly causes genital lesions, whereas HSV-1 is involved in both oral and genital infections. Glycoprotein D (gD) is a structural component of the herpes simplex virus type 1 (HSV-1) envelope which is essential for virus entry and fusion with host cells. gD plays an important role by binding to the host receptors such as herpes virus entry mediator (HVEM) and nectin-1, a member of the immunoglobulin (Ig)-like cell adhesion molecules.
• Description: HSV-2 (strain 333) Envelope Glycoprotein D (gD), His Tag (GLD-V52H4) is expressed from human 293 cells (HEK293). It contains AA Lys 26 - Tyr 310 (Accession # P03172-1).
• Specifications: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 33.7 kDa. The protein migrates as 44-47 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression Region: Lys 26 - Tyr 310
• Host: HEK 293
• Target: Glycoprotein D/gD (HSV)
• Conjugation: Unconjugated
• Tag: C-10×His
• Source: HSV-2
• Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Endotoxin: 1.0 EU per μg
• Purity: 95%
• Bioactivity: Immobilized HSV-2 (strain 333) Envelope Glycoprotein D (gD), His Tag (Cat. No. GLD-V52H4) at 5 μg/mL (100 μL/well) can bind Human Nectin-1, Fc Tag (Cat. No. PV1-H5253) with a linear range of 0.2-5 ng/mL (QC tested).
• Format: Powder
• Buffer: PBS, pH7.4
• Additives: Trehalose
• Molecular Weight: 33.7 kDa
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C