Human CD133 Protein, His Tag (Detergent)

• Background:

  Prominin-1 is also known as CD133, Antigen AC133, PROM1, PROML1 and MSTP061. Is used as marker for hematopoietic stem and progenitor cells (HSPC) for somatic stem cell isolation. May play a role in cell differentiation, proliferation and apoptosis . Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner.

• Description:

  Prominin-1 is also known as CD133, Antigen AC133, PROM1, PROML1 and MSTP061. Is used as marker for hematopoietic stem and progenitor cells (HSPC) for somatic stem cell isolation. May play a role in cell differentiation, proliferation and apoptosis . Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner.

• Specifications:

  This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 97.2 kDa. The protein migrates as 90-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

• Accession Number:

  O43490-1

• Expression Region:

  Gly 20 - His 865

• Host:

  HEK293

• Target:

  CD133

• Conjugation:

  Unconjugated

• Tag:

  C-10xHis

• Source:

  Human

• Stability:

  The product MUST be stored at -70°C or lower upon receipt; -70°C for 3 months under sterile conditions.

• Endotoxin:

  1.0 EU per μg

• Purity:

  90%

• Bioactivity:

  Immobilized Human CD133, His Tag (Cat. No. CD3-H52H4) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CD133 Antibody, Human IgG1 with a linear range of 1-16 ng/mL (QC tested).

• Format:

  Liquid

• Buffer:

  50 mM HEPES, 150 mM NaCl, DDM, CHS, pH7.5

• Additives:

  Glycerol

• Molecular Weight:

  97.2 kDa

• Additionnal Information:

  Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments

• Shipping Conditions:

  Dry ice

• Storage Conditions:

  -70°C

• Package Size:

  20ug*1

• Host or Source:

  HEK293

• Species:

  Human

• Protein ID:

  O43490-1

• Preservative:

  Glycerol