Biotinylated Human HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Complex Protein (Monomer, MALS verified)

Biotinylated Human HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Complex Protein (Monomer, MALS verified)

• Background: Cytomegalovirus (Cytomegalovirus) is a herpes viral DNA virus. Human cytomegalovirus (HCMV) can only infect humans and multiply in humans. The antibodies. After primary infection with CMV, the body can produce specific antibodies and killer T lymphocytes to activate NM cells. The antibody has limited CMV replication ability and has a certain resistance to reinfection of the same strain, but cannot resist the activation of antibody-dependent virus and the exogenous infection of other different strains of CMV. However, specific killer T lymphocytes and antibody-dependent cytotoxic cells can exert the greatest antiviral effect. The Human HLA-A*0201 CMV (NLVPMVATV) complex protein is a complex of HLA-A*0201 of the MHC Class I, B2M, and NLVPMVATV peptide of the CMV.
• Description: Biotinylated Human HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Complex Protein (HLC-H82E5) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Ile 308 (HLA-A*02:01) & Ile 21 - Met 119 (B2M) & NLVPMVATV peptide (Accession # AAA59606.1 (HLA-A*02:01) & P61769 (B2M) & NLVPMVATV).
• Specifications: Biotinylated Human HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Complex Protein is produced by co-expression of HLA and B2M loaded with CMV pp65 peptide. This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 36.3 kDa and 11.7 kDa. The protein migrates as 43-48 kDa and 10 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression Region: Gly 25 - Ile 308 (HLA-A*02:01) & Ile 21 - Met 119 (B2M) & NLVPMVATV peptide
• Host: HEK 293
• Target: HLA-A*0201 & B2M & CMV pp65
• Conjugation: Biotin-labeled
• Tag: C-10×His & C-Avi
• Source: Human
• Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Endotoxin: 1.0 EU per μg
• Purity: 90%
• Bioactivity: Immobilized Biotinylated Human HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Complex Protein (Cat. No. HLC-H82E5) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-HLA-A2&CMV pp65 Antibody, Human IgG1 with a linear range of 0.1-10 ng/mL (QC tested).
• Format: Powder
• Buffer: PBS, pH7.4
• Additives: Trehalose
• Molecular Weight: 36.3 kDa and 11.7 kDa
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C