Biotinylated Human HLA-A*02:01&B2M&HPV (TIHDIILECV) Complex Protein (Monomer, MALS verified)

Biotinylated Human HLA-A*02:01&B2M&HPV (TIHDIILECV) Complex Protein (Monomer, MALS verified)

• Background: Human papillomavirus (HPV) is A kind of papillomavirus belonging to the milk polypoid virus family. It is a spherical D virus, which can cause the proliferation of squamous epithelium of human skin mucosa. HPV(human papillomavirus) for common warts, genital warts (condyloma acuminatum), and other symptoms. There are many types of human papillomavirus (HPV), with HPV 16 and 18 being high-risk types known to significantly increase the risk of cervical, vaginal and vulvar cancers in women and men. The Biotin human HLA-A*0201 HPV (TIHDIILECV) protein is a complex of HLA-A*0201 of the MHC Class I, B2M, and TIHDIILECV peptide of the HPV.
• Description: Biotinylated Human HLA-A*02:01&B2M&HPV (TIHDIILECV) Complex Protein (HLV-H82E5) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Ile 308 (HLA-A*02:01) & Ile 21 - Met 119 (B2M) & TIHDIILECV peptide (Accession # AAA59606.1 (HLA-A*02:01) & P61769-1 (B2M) & TIHDIILECV).
• Specifications: Biotinylated Human HLA-A*02:01&B2M&HPV (TIHDIILECV) Complex Protein is produced by co-expression of HLA and B2M loaded with HPV peptide. This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 36.3 kDa and 13.9 kDa. The protein migrates as 40-43 kDa and 12 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression Region: Gly 25 - Ile 308 (HLA-A*02:01) & Ile 21 - Met 119 (B2M) & TIHDIILECV peptide
• Host: HEK 293
• Target: HLA-A*0201 & B2M & HPV (TIHDIILECV)
• Conjugation: Biotin-labeled
• Tag: C-10×His & C-Avi
• Source: Human
• Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Endotoxin: 1.0 EU per μg
• Purity: 90%
• Bioactivity: Immobilized Biotinylated Human HLA-A*02:01&B2M&HPV (TIHDIILECV) Complex Protein (Cat. No. HLV-H82E5) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.06-1 ng/mL (QC tested).
• Format: Powder
• Buffer: PBS, pH7.4
• Additives: Trehalose
• Molecular Weight: 36.3 kDa and 13.9 kDa
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C