Biotinylated Cynomolgus Complement C2 Protein, His,Avitag™ (active enzyme, MALS verified)

Biotinylated Cynomolgus Complement C2 Protein, His,Avitag™ (active enzyme, MALS verified)

• Background: C2 is a major histocompatibility complex class-III protein. Component C2 which is part of the classical pathway of the complement system is cleaved by activated factor C1 into two fragments: C2b and C2a. C2a, a serine protease, then combines with complement factor C4b to generate the C3 or C5 convertase. The lectin (LP) and classical (CP) pathways are two of the three main activation cascades of the complement system. These pathways start with recognition of different pathogen- or danger-associated molecular patterns and include identical steps of proteolytic activation of complement component C4, formation of the C3 proconvertase C4b2, followed by cleavage of complement component C2 within C4b2 resulting in the C3 convertase C4b2a.
• Description: Biotinylated Cynomolgus Complement C2 Protein, His,Avitag (CO2-C82E7) is expressed from human 293 cells (HEK293). It contains AA Ala 21 - Leu 752 (Accession # G8F3W0).
• Specifications: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 84.7 kDa. The protein migrates as 95-115 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression Region: Ala 21 - Leu 752
• Host: HEK 293
• Target: Complement C2
• Conjugation: Biotin-labeled
• Tag: C-10×His & C-Avi
• Source: Cynomolgus
• Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Endotoxin: 1.0 EU per μg
• Purity: 90%
• Format: Powder
• Buffer: 20 mM Tris, 150 mM NaCl, pH7.5
• Additives: Trehalose
• Molecular Weight: 84.7 kDa
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C