Cynomolgus MMP-9 Protein, His Tag (active enzyme)

• Background:

  Matrix metallopeptidase 9 (MMP-9) is also known as 92 kDa type IV collagenase, 92 kDa gelatinase or gelatinase B (GELB), CLG4B, is secreted from neutrophils, macrophages, and a number of transformed cells, and is the most complex family member in terms of domain structure and regulation of its activity. . Structurally, MMP9 maybe be divided into five distinct domains: a prodomain which is cleaved upon activation, a gelatinbinding domain consisting of three contiguous fibronectin type II units, a catalytic domain containing the zinc binding site, a prolinerich linker region, and a carboxyl terminal hemopexinlike domain. This enzyme degrades various substrates including gelatin, collagen types IV and V, and elastin. MMP9 is involved in a variety of autoimmune diseases such as systemic lupus erythematosus, rheumatoid arthritis, and multiple sclerosis, and be regarded as a potential therapeutic target.

• Description:

  Cynomolgus MMP-9 Protein, His Tag (MM9-C52H3) is expressed from human 293 cells (HEK293) . It contains AA Ala 20 - Pro 469 (Accession # XP_005569271.2) . It needs to be activated by agents such as APMA in vitro to have hydrolytic activity.

• Specifications:

  This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 51.8 kDa. The protein migrates as 65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

• Accession Number:

  XP_005569271.2

• Expression Region:

  Ala 20 - Pro 469

• Host:

  HEK293

• Target:

  MMP-9

• Conjugation:

  Unconjugated

• Tag:

  C-10xHis

• Source:

  Cynomolgus

• Stability:

  ● The product MUST be stored at -70°C or lower upon receipt; ● -70°C for 3 months under sterile conditions.

• Endotoxin:

  1.0 EU per μg

• Purity:

  90%

• Format:

  Liquid

• Buffer:

  25 mM Tris, 150 mM NaCl, pH7.5

• Additives:

  Glycerol

• Molecular Weight:

  51.8 kDa

• Shipping Conditions:

  Dry ice

• Storage Conditions:

  -70°C

• Package Size:

  50ug*1

• Species:

  Cynomolgus