Human Coagulation factor XI / FXI Protein, His Tag (active enzyme)

Human Coagulation factor XI / FXI Protein, His Tag (active enzyme)

• Background: Coagulation factor XI is also known as F11, PTA and FXI. Factor XI triggers the middle phase of the intrinsic pathway of blood coagulation by activating factor IX. Factor XI is synthesized in the liver and circulates in the plasma as a disulfide bond-linked dimer complexed with high molecular weight kininogen. Selective cleavage of Arg-|-Ala and Arg-|-Val bonds in factor IX to form factor IXa. Factor XI is converted into XIa via either the contact phase of blood coagulation or thrombin-mediated activation on the platelet surface. The resulting XIa converts factor IX into IXa, which subsequently activates factor X into Xa.
• Description: Human Coagulation factor XI, His Tag (FXI-H52H5) is expressed from human 293 cells (HEK293). It contains AA Glu 19 - Val 625 (Accession # P03951-1).
• Specifications: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 69.9 kDa. The protein migrates as 35 kDa and 50 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
• Expression Region: Glu 19 - Val 625
• Host: HEK 293
• Target: Coagulation factor XI
• Conjugation: Unconjugated
• Tag: C-10×His
• Source: Human
• Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Endotoxin: 1.0 EU per μg
• Purity: 90%
• Bioactivity: Immobilized Human Coagulation factor XI, His Tag (Cat. No. FXI-H52H5) at 1 μg/mL (100 μL/well) can bind Abelacimab with a linear range of 0.2-8 ng/mL (QC tested).
• Format: Powder
• Buffer: 20 mM HEPES, 150 mM NaCl, pH7.5
• Additives: Trehalose
• Molecular Weight: 69.9 kDa
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C