Human Coagulation Factor III / Tissue Factor Protein, Fc Tag (active enzyme)

Human Coagulation Factor III / Tissue Factor Protein, Fc Tag (active enzyme)

• Background: Full-length tissue factor (TF) is a transmembrane receptor and cofactor for factor (F)VII/FVIIa. In addition to full-length TF, an alternative spliced (as) form of TF can be generated that lacks the transmembrane domain and is released fromcells. In contrast to TF, asTF has low procoagulant activity because it lacks the transmembrane domain. Tissue factor is expressed by cells around blood vessels, such as adventitial fibroblasts, and body surfaces, such as epithelial cells, and plays a critical role in hemostasis. TF also contributes to various forms of thrombosis. Many cancers, particularly adenocarcinomas, express high levels of TF. A high level of tumor TF expression is associated with poor prognosis in many types of cancers, including breast, prostate, colorectal, and pancreatic cancer.
• Description: Human Tissue Factor, Fc Tag (TF3-H5253) is expressed from human 293 cells (HEK293). It contains AA Ser 33 - Glu 251 (Accession # P13726-1).
• Specifications: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 51.2 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression Region: Ser 33 - Glu 251
• Host: HEK 293
• Target: Coagulation Factor III
• Conjugation: Unconjugated
• Tag: C-Fc
• Source: Human
• Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Endotoxin: 1.0 EU per μg
• Purity: 95%
• Bioactivity: Immobilized Human Tissue Factor, Fc Tag (Cat. No. TF3-H5253) at 1 μg/mL (100 μL/well) can bind Anti-TF Antibody, Human IgG1 with a linear range of 0.2-2 ng/mL (Routinely tested).
• Format: Powder
• Buffer: 50 mM Tris, 150 mM NaCl, pH7.5
• Additives: Trehalose
• Molecular Weight: 51.2 kDa
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C