Mouse Coagulation factor VII Protein, His Tag (active enzyme, MALS verified)

Mouse Coagulation factor VII Protein, His Tag (active enzyme, MALS verified)

• Background: Coagulation factor VII is also known as F7, SPCA and Proconvertin. Heterodimer of a light chain and a heavy chain linked by a disulfide bond. Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium.
• Description: Mouse Coagulation factor VII, His Tag (COI-M52H1) is expressed from human 293 cells (HEK293). It contains AA Ala 42 - Leu 446 (Accession # P70375-1).
• Specifications: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 47.7 kDa. The protein migrates as 57-62 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression Region: Ala 42 - Leu 446
• Host: HEK 293
• Target: Coagulation factor VII
• Conjugation: Unconjugated
• Tag: C-10×His
• Source: Mouse
• Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Endotoxin: 1.0 EU per μg
• Purity: 90%
• Format: Powder
• Buffer: 50 mM Tris,150 mM Nacl,pH8.0
• Additives: Trehalose
• Molecular Weight: 47.7 kDa
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C