Recombinant Streptococcus pyogenes Exotoxin type A (speA)

Recombinant Streptococcus pyogenes Exotoxin type A (speA)

• CAS Number: 9000-83-3
• Gene Name: speA
• UniProt: P0DJY7
• Expression Region: 31-250aa
• Organism: Streptococcus pyogenes
• Target Sequence: STRPKPSQLQRSNLVKTFKIYIFFMRVTLVTHENVKSVDQLLSHDLIYNVSGPNYDKLKTELKNQEMATLFKDKNVDIYGVEYYHLCYLCENAERSACLYGGVTNHEGNHLEIPKKIVVKVSIDGIQSLSFDIEQIKNGNCSRISYTVRKYLTDNKQLYTNGPSKYETGYIKFIPKNKESFWFDFFPEPEFTQSKYLMIYKDNETLDSNTSQIEVYLTTK
• Tag: N-terminal 6xHis-SUMO-tagged
• Source: E.coli
• Field of Research: Others
• Assay Type: In Stock Protein
• Relevance: Causative agent of the symptoms associated with scarlet fever, have been associated with streptococcal toxic shock-like disease and may play a role in the early events of rheumatic fever.
• Purity: Greater than 85% as determined by SDS-PAGE.
• Activity: Not test
• Length: Full Length of Mature Protein
• Form: Liquid or Lyophilized powder
• Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Molecular Weight: 38.6 kDa
• References & Citations: "Crystal structure of a dimeric form of streptococcal pyrogenic exotoxin A (SpeA1)." Baker M.D., Gendlina I., Collins C.M., Acharya K.R. Protein Sci 13:2285-2290 (2004).
• Storage Conditions: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.