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Recombinant Streptococcus pyogenes Streptopain (speB)

CAT: 0399-CSB-EP314886SMR-01Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0399-CSB-EP314886SMR-01Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
Exotoxin type B SPE B Streptococcal cysteine proteinase Streptococcus peptidase A Short name: SPP
Abbreviation
Recombinant Streptococcus pyogenes speB protein
Gene Name
SpeB
UniProt
P0C0J0
Expression Region
146-398aa
Organism
Streptococcus pyogenes
Target Sequence
QPVVKSLLDSKGIHYNQGNPYNLLTPVIEKVKPGEQSFVGQHAATGCVATATAQIMKYHNYPNKGLKDYTYTLSSNNPYFNHPKNLFAAISTRQYNWNNILPTYSGRESNVQKMAISELMADVGISVDMDYGPSSGSAGSSRVQRALKENFGYNQSVHQINRSDFSKQDWEAQIDKELSQNQPVYYQGVGKVGGHAFVIDGADGRNFYHVNWGWGGVSDGFFRLDALNPSALGTGGGAGGFNGYQSAVVGIKP
Tag
N-terminal 6xHis-SUMO-tagged
Type
In Stock Protein
Source
E.coli
Field of Research
Microbiology
Relevance
Important streptococcal virulence factor which cleaves human fibronectin and degrades vitronectin. Also cleaves human IL1B precursor to form biologically active IL1B. Can induce apoptosis in human monocytes and epithelial cells in vitro, and reduces phagocytic activity in monocytic cells. Thus, may play a role in bacterial colonization, invasion, and inhibition of wound healing
Endotoxin
Not test
Purity
Greater than 90% as determined by SDS-PAGE.
Activity
Not Test
Form
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Function
Important streptococcal virulence factor which cleaves human fibronectin and degrades vitronectin. Also cleaves human IL1B precursor to form biologically active IL1B. Can induce apoptosis in human monocytes and epithelial cells in vitro, and reduces phagocytic activity in monocytic cells. Thus, may play a role in bacterial colonization, invasion, and inhibition of wound healing.
Molecular Weight
43.6 kDa
References & Citations
"A conserved Streptococcus pyogenes extracellular cysteine protease cleaves human fibronectin and degrades vitronectin."Kapur V., Topouzis S., Majesky M.W., Li L.L., Hamrick M.R., Hamill R.J., Patti J.M., Musser J.M.Microb. Pathog. 15:327-346 (1993)
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Protein Length
Full Length of Mature Protein

UniProtKB · P0C0J0

Streptopain

SPEB_STRPY · Streptococcus pyogenes

View on UniProt ↗
Primary accession
P0C0J0
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
speB
Protein existence
1: Evidence at protein level
Organism
Streptococcus pyogenes
Taxonomy ID
1314
Alternative names
—
EC number
3.4.22.10
Processing
Precursor
Secondary accessions
P00788, P26296, P68883, Q54960, Q54961, Q54962, Q54963, Q54964, Q54965, Q54966, Q54967, Q54968, Q57024, Q57082, Q57202, Q57211, Q57212, Q9S680
Protein keywords

Technical term

3D-structureDirect protein sequencing

PTM

Autocatalytic cleavageMethylationZymogen

Cellular component

Host cytoplasmSecreted

Molecular function

HydrolaseProteaseThiol proteaseToxin

Domain

Signal

Biological process

Virulence

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