Anti-Heme Oxygenase-1 Antibody

• Description:

  Mouse Monoclonal Antibody specific to Heme Oxygenase-1

• Product Name Alternative:

  HO-1, EC 1.14.14.18

• Gene Name:

  HMOX1

• NCBI Gene ID:

  HMOX1

• UniProt:

  P09601

• Accession Number:

  NP_002124.1

• Cellular Locus:

  Microsome, Endoplasmic reticulum membrane, Peripheral membrane protein, Cytoplasmic side

• Host:

  Mouse

• Reactivity:

  Human, Mouse, Bovine

• Immunogen:

  Synthetic peptide corresponding to aa 1-30 of human HO-1.

• Target Antigen:

  Heme oxygenase 1

• Target:

  Heme Oxygenase-1

• Clonality:

  Monoclonal

• Isotype:

  IgG1

• Type:

  Antibody

• Applications:

  WB, ELISA

• Field of Research:

  Enzymes

• Purification Method:

  Purified by Protein G affinity chromatography

• Concentration:

  1.0 mg/mL

• Dilution:

  Dilute in PBS or medium that is identical to that used in the assay system.

• Format:

  Purified

• Form:

  Liquid

• Buffer:

  Phosphate Buffered Saline

• Function:

  Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Exhibits cytoprotective effects since excess of free heme sensitizes cells to undergo apoptosis.

• Additionnal Information:

  ELISA: use at 1ug/ml with HO-1 on the solid phase.
  
  Immunoblotting: use at 0.5-1ug/ml. A band of ~32kDa is detected.
  
  Immunofluorescence: use at 5-10ug/ml
  
  Positive control: Human cell line lysates such as A431, A549, HeLa, HepG2, Jurkat, or MCF7.
  
  These are recommended concentrations.
  
  Enduser should determine optimal concentrations for their applications.

• Storage Conditions:

  This antibody is stable for at least one (1) year at -20°C.

• Specificity:

  This antibody recognizes human, mouse, and bovine HO-1. It does not cross-react with HO-2.

• Formulation:

  PBS, pH 7.4.

• Buffer pH:

  pH 7.4

• Target Background:

  Heme-oxygenase is an enzyme that catalyzes heme catabolism to yield biliverdin, iron, and carbon monoxide (CO). There are three isoforms of heme-oxygenase: HO-1, HO-2, and HO-3. HO-1 and HO-2 have been identified as the two major isoforms in mammals. HO-1, also known as heat shock protein 32 (Hsp32) is induced by most oxidative stress inducers, cytokines, inflammatory agents, and heat shock. HO-1 deficiency appears to cause reduced stress defense, a pro-inflammatory tendency, susceptibility to atherosclerotic lesion formation, endothelial cell injury, and growth retardation. Therefore, up-regulation of HO-1 is one of the major defense mechanisms against oxidative stress.

• CAS Number:

  9007-83-4