Recombinant Human RUNX3 protein (His tag)

• Background:

  RUNX3, also called CBFA3, AML-2 or PEBP2-alpha C, is a member of the Runt domain family of nuclear transcriptional regulators. All of the RUNX proteins form dimers with CBF-beta. The runt domain (aa 54-186) is required for DNA binding, while a pro/ser/thr-rich region (aa 191-415) transcriptionally activates target genes. Isoform 2 has an alternate 19 aa in place of the N-terminal 5 aa of isoform 1. The 415 aa Human RUNX3 shares 91% aa identity with mouse or rat RUNX3. RUNX3 is necessary for growth control of gastric epithelium, neurogenesis of dorsal root ganglia, and T cell differentiation. RUNX3 expression is frequently mutated in tumors and appears to be silenced by methylation.

• Abbreviation:

  RUNX3

• Synonyms:

  Acute myeloid leukemia 2 protein; Core-binding factor subunit alpha-3; Oncogene AML-2; RUNX3; Runt-related transcription factor 3

• UniProt:

  Q13761

• Accession Number:

  Q13761

• Expression System:

  E.coli

• Tag:

  N-His

• Sequence:

  Met 1-Tyr 415

• Field of Research:

  Epigenetics and Nuclear signaling; Neuroscience

• Endotoxin:

  < 10 EU/mg of the protein as determined by the LAL method.

• Purity:

  > 95% as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  45.5 kDa

• Observed Molecular Weight:

  50 kDa

• Species:

  Human