Phospho-SQSTM1 / p62 (S349) Recombinant Rabbit Monoclonal Antibody [PSH08-97]

Phospho-SQSTM1 / p62 (S349) Recombinant Rabbit Monoclonal Antibody [PSH08-97]

• Description: Autophagy receptor required for selective macroautophagy (aggrephagy). Functions as a bridge between polyubiquitinated cargo and autophagosomes. Interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family. Along with WDFY3, involved in the formation and autophagic degradation of cytoplasmic ubiquitin-containing inclusions (p62 bodies, ALIS/aggresome-like induced structures). Along with WDFY3, required to recruit ubiquitinated proteins to PML bodies in the nucleus. May regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. Adapter that mediates the interaction between TRAF6 and CYLD. May be involved in cell differentiation, apoptosis, immune response and regulation of K+ channels. Involved in endosome organization by retaining vesicles in the perinuclear cloud: following ubiquitination by RNF26, attracts specific vesicle-associated adapters, forming a molecular bridge that restrains cognate vesicles in the perinuclear region and organizes the endosomal pathway for efficient cargo transport. Promotes relocalization of 'Lys-63'-linked ubiquitinated STING1 to autophagosomes. Acts as an activator of the NFE2L2/NRF2 pathway via interaction with KEAP1: interaction inactivates the BCR (KEAP1) complex, promoting nuclear accumulation of NFE2L2/NRF2 and subsequent expression of cytoprotective genes.
• Product Name Alternative: A170 antibody DMRV antibody EBI 3 associated protein of 60 kDa antibody EBI 3 associated protein p60 antibody EBI3 associated protein of 60 kDa antibody EBI3 associated protein p60 antibody EBI3-associated protein of 60 kDa antibody EBIAP antibody FTDALS3 antibody MGC127197 antibody ORCA antibody OSF-6 antibody Osi antibody OSIL antibody Oxidative stress induced like antibody p60 antibody p62 antibody p62B antibody Paget disease of bone 3 antibody PDB 3 antibody PDB3 antibody Phosphotyrosine independent ligand for the Lck SH2 domain of 62 kDa antibody Phosphotyrosine independent ligand for the Lck SH2 domain p62 antibody Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa antibody PKC-zeta-interacting protein antibody Protein kinase C-zeta-interacting protein antibody Sequestosome 1 antibody Sequestosome-1 antibody SQSTM 1 antibody SQSTM_HUMAN antibody Sqstm1 antibody STAP antibody STONE14 antibody Ubiquitin binding protein p62 antibody Ubiquitin-binding protein p62 antibody ZIP 3 antibody ZIP antibody ZIP3 antibody
• Abbreviation: Sequestosome-1 EBI3-associated protein of 60 kDa (EBIAP; p60) Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa Ubiquitin-binding protein p62 SQSTM1 ORCA OSIL
• CAS Number: 9000-83-3
• Swiss Prot: Q13501 Human, Q64337 Mouse
• Cellular Locus: Cytoplasm, Endoplasmic reticulum, Endosome, Lysosome, Nucleus.
• Host: Rabbit
• Species Reactivity: Human, Mouse
• Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser349 of Human SQSTM1/ p62.
• Isotype: IgG
• Clone: PSH08-97
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IF-Cell, IHC-P
• Positive Control: HeLa treated with 2μM MG-132 for 18 hours cell lysate, NIH/3T3 treated with 10μM MG-132 for 8 hours cell lysate, HeLa cells treated with 2μM MG-132 for 18 hours, NIH/3T3cells treated with 10μM MG-132 for 8 hours , human cerebrum tissue.
• Concentration: 1 mg/mL
• Dilution: WB: 1:2,000-1:5,000 ;IF-Cell: 1:5,000 ;IHC-P: 1:30,000
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 48 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
• Recombinant Antibody: Yes