Human Alpha-Synuclein (E46K) Protein, Tag Free (MALS verified)

• Background:

  Alpha-synuclein is a neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. It acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5. Abnormalities in alpha-synuclein are implicated in the pathogenesis of Parkinson's disease (PD) . Alpha-synuclein is present in Lewy-bodies, the neuropathological hallmark of PD, and the protein and its aggregation have been widely linked to neurotoxic pathways that ultimately lead to neurodegeneration.

• Description:

  Human Alpha-Synuclein (E46K), Tag Free (ALN-H5117) is expressed from E. coli cells. It contains AA Met 1 - Ala 140 (Accession # P37840-1 (E46K) ) .

• Specifications:

  This protein carries no "tag". The protein has a calculated MW of 14.5 kDa. The protein migrates as 16 kDa under reducing (R) condition (SDS-PAGE) .

• Accession Number:

  P37840-1 (E46K)

• Expression Region:

  Met 1 - Ala 140

• Host:

  E. coli

• Target:

  Alpha-Synuclein

• Conjugation:

  Unconjugated

• Tag:

  Native

• Source:

  Human

• Stability:

  ● The product MUST be stored at -70°C or lower upon receipt; ● -70°C for 3 months under sterile conditions.

• Endotoxin:

  1.0 EU per μg

• Purity:

  95%

• Bioactivity:

  Immobilized Human Alpha-Synuclein (E46K), Tag Free (Cat. No. ALN-H5117) at 1 μg/mL (100 μL/well) can bind Synuclein alpha/beta Monoclonal Antibody with a linear range of 0.1-4 ng/mL (QC tested).

• Format:

  Liquid

• Buffer:

  50mM HEPES, 100mM NaCl, pH8.0

• Additives:

  Trehalose

• Molecular Weight:

  14.5 kDa

• Shipping Conditions:

  Dry ice

• Storage Conditions:

  -70°C

• Package Size:

  200ug*1

• Species:

  Human