Biotinylated Human Alpha-Synuclein Protein, His, Avitag™

• Background:

  Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores. Mechanistically, acts by increasing local Ca2+ release from microdomains which is essential for the enhancement of ATP-induced exocytosis. Acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5. This chaperone activity is important to sustain normal SNARE-complex assembly during aging. Plays also a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity.

• Description:

  Biotinylated Human Alpha-Synuclein, His, Avitag (ALN-H82H8) is expressed from E. coli cells. It contains AA Met 1 - Ala 140 (Accession # P37840-1) .

• Specifications:

  This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 18.0 kDa. The protein migrates as 23 kDa under reducing (R) condition (SDS-PAGE) .

• Accession Number:

  P37840-1

• Expression Region:

  Met 1 - Ala 140

• Host:

  E. coli

• Target:

  Alpha-Synuclein

• Conjugation:

  Biotin-labeled

• Tag:

  C-10xHis & C-Avi

• Source:

  Human

• Stability:

  ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

• Endotoxin:

  1.0 EU per μg

• Purity:

  95%

• Format:

  Powder

• Buffer:

  100 mM NaAC, pH7.0

• Additives:

  Trehalose

• Molecular Weight:

  18.0 kDa

• Shipping Conditions:

  RT

• Storage Conditions:

  -20°C

• Package Size:

  200ug*1

• Species:

  Human