Mouse anti-active Caspase 3 Antibody

• Background:

  Mouse monoclonal antibody to active Caspase 3

• Synonyms:

  CPP32; Caspase-3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1

• Host:

  Mouse

• Reactivity:

  H, M, R

• Immunogen:

  Recombinant protein corresponding to human active Caspase 3.

• Clonality:

  Monoclonal

• Applications:

  WB, IHC

• Concentration:

  1mg/mL

• Dilution:

  WB (1/500 - 1/1000), IH (1/100 - 1/200)

• Buffer:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

• Storage Conditions:

  Store at -20°C. Avoid repeated freeze / thaw cycles.

• Host or Source:

  Mouse

• WB Description:

  Western blot analysis of active Caspase 3 expression in Hela (A), NIH3T3 (B), rat brain (C) whole cell lysates.

• IHC description:

  Immunohistochemical analysis of active Caspase 3 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• Gene ID (Human):

  836

• SwissProt (Human):

  P42574

• CAS Number:

  9007-83-4