Rabbit anti-SCF Antibody

• Background:

  Rabbit polyclonal antibody to SCF

• Synonyms:

  MGF; SCF; Kit ligand; Mast cell growth factor; MGF; Stem cell factor; SCF; c-Kit ligand

• Host:

  Rabbit

• Reactivity:

  H

• Immunogen:

  Recombinant full length protein of human SCF

• Clonality:

  Polyclonal

• Applications:

  WB, IHC

• Purification Method:

  The antibody was purified by immunogen affinity chromatography.

• Concentration:

  1 mg/mL

• Dilution:

  WB (1/500 - 1/2000), IH (1/50 - 1/100)

• Buffer:

  Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3,30% glycerol, and 0.01% sodium azide.

• Storage Conditions:

  Store at -20°C. Avoid repeated freeze / thaw cycles.

• WB Description:

  Western blot analysis of SCF expression in HT29 (A), HepG2 (B), Raji (C) whole cell lysates.

• IHC description:

  Immunohistochemical analysis of SCF staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• Gene ID (Human):

  4254

• SwissProt (Human):

  P21583

• CAS Number:

  9007-83-4