Anti-SCF Rabbit Monoclonal Antibody

• Description:

  Boster Bio Anti-SCF Rabbit Monoclonal Antibody catalog # M01254. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.

• Synonyms:

  Kit ligand; Mast cell growth factor; MGF; Stem cell factor; SCF; c-Kit ligand; Soluble KIT ligand; sKITLG; KITLG; MGF, SCF

• Gene Name:

  Kit ligand

• UniProt:

  P21583

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A synthesized peptide derived from human SCF

• Clonality:

  Monoclonal

• Clone:

  Clone: FAG-11

• Tissue Specificity:

  The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.

• Applications:

  WB, IHC, ICC, IF, Flow Cytometry

• Field of Research:

  Cytokines, Developmental Biology, Embryogenesis, Embryonic Stem Cells, Immunology, Innate Immunity, Stem Cells, Surface Molecules

• Purification:

  Affinity-chromatography

• Concentration:

  Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

• Form:

  Liquid

• Reconstitution:

  Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

• Function:

  Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5- trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.

• Storage Conditions:

  Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

• Fragment:

  Rabbit IgG

• Applications Notes:

  WB 1:500-1:2000
  IHC 1:50-1:200
  ICC/IF 1:50-1:200
  FC 1:20

• Other Gene Names:

  KITLG

• Subcellular Location:

  Isoform 1: Cell membrane; Single-pass type I membrane protein.

• Protein Name:

  Glycogen synthase kinase-3 beta (gsk3b)